Moskowitz R W, Howell D S, Goldberg V M, Muniz O, Pita J C
Arthritis Rheum. 1979 Feb;22(2):155-63. doi: 10.1002/art.1780220208.
Size distribution of cartilage proteoglycans (PG) extracted from control and osteoarthritic rabbit knees after partial meniscectomy was analyzed. In normal control knees, about 30% of PG molecules were in aggregate form and average sedimentation constant was 60S. No aggregates were found in osteoarthritic cartilage, whether ulcer, rim about ulcer, or distant normal-appearing cartilage was examined. Weight average sedimentation constants for PG subunits were similar to controls, 15S. Up to 70% of guanidinium-extractable PG could be extracted from osteoarthritic cartilage by using 0.5M guanidine HC1 (GuHCl); sedimentation characteristics of extracted PG were similar to those using 4.0M GuHCl. Absence of aggregates is consistent with a disorder of link protein, hyaluronic acid, or PG subunit hyaluronic acid binding sites. Absence of subunit degradation was an enexpected finding. The demonstrated ability of 0.5 M GuHCl to extract large amounts of PG from osteoarthritic cartilage will allow study of cartilage proteoglycans in their native nondissociated state.
分析了部分半月板切除术后从对照和骨关节炎兔膝关节中提取的软骨蛋白聚糖（PG）的大小分布。在正常对照膝关节中，约30%的PG分子呈聚集形式，平均沉降常数为60S。在骨关节炎软骨中未发现聚集物，无论是溃疡处、溃疡边缘还是远处外观正常的软骨均如此。PG亚基的重均沉降常数与对照相似，为15S。通过使用0.5M盐酸胍（GuHCl），高达70%的胍可提取PG能从骨关节炎软骨中提取出来；提取的PG的沉降特性与使用4.0M GuHCl时相似。聚集物的缺失与连接蛋白、透明质酸或PG亚基透明质酸结合位点的紊乱一致。亚基未降解是一个意外发现。0.5M GuHCl从骨关节炎软骨中提取大量PG的能力将有助于在其天然未解离状态下研究软骨蛋白聚糖。
