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1
Coupling of Escherichia coli lipopolysaccharide to epoxy-activated Sepharose 6B.大肠杆菌脂多糖与环氧活化的琼脂糖凝胶6B的偶联
Infect Immun. 1978 Jun;20(3):867-8. doi: 10.1128/iai.20.3.867-868.1978.
2
[Coupling of bovine gamma globulin to carbonochloridate-activated cellulose beads, CNBr-activated Sepharose CL-4B and NaIO4-oxidized Sepharose 6B and use of the coupled products for immunoaffinity chromatography].[牛γ球蛋白与氯甲酸酯活化纤维素珠、溴化氰活化琼脂糖凝胶CL-4B及高碘酸钠氧化琼脂糖凝胶6B的偶联及其偶联产物在免疫亲和色谱中的应用]
Allerg Immunol (Leipz). 1990;36(4):299-307.
3
[Properties of immunoadsorbents prepared by antigen coupling to glutaraldehydeactivated polyacrylamide gel, BrCN-activated Sepharose and by copolymerization of antigens by glutaraldehyde].[通过抗原偶联到戊二醛活化的聚丙烯酰胺凝胶、溴化氰活化的琼脂糖以及通过戊二醛进行抗原共聚制备的免疫吸附剂的性质]
Ukr Biokhim Zh (1978). 1978 Jul-Aug;50(4):500-11.
4
[Preparation of immunosorbents from lipopolysaccharides and polysaccharides extracted from various gram-negative and gram-positive bacteria].
Ann Immunol (Paris). 1982 Nov-Dec;133D(3):327-34.
5
Use of stable 6-aminohexyl derivatives for labelling polysaccharides with haptens and for preparing polysaccharide immunoadsorbents.使用稳定的6-氨基己基衍生物用半抗原标记多糖并制备多糖免疫吸附剂。
J Immunol Methods. 1993 Mar 15;160(1):135-7. doi: 10.1016/0022-1759(93)90017-2.
6
A novel preparation of immunoadsorbents. Controlled coupling of proteins to Sepharose-gelatin by heterobifunctional reagent.免疫吸附剂的一种新制备方法。通过异双功能试剂将蛋白质可控偶联至琼脂糖-明胶上。
Biochim Biophys Acta. 1981 Apr 3;673(4):527-38. doi: 10.1016/0304-4165(81)90484-0.
7
Magnetically responsive polyacrylamide agarose beads for the preparation of immunoabsorbents.用于制备免疫吸附剂的磁响应性聚丙烯酰胺琼脂糖珠
J Immunol Methods. 1978;21(1-2):59-63. doi: 10.1016/0022-1759(78)90223-5.
8
A method for preparation of immunosorbents by direct coupling of dissociated immune complexes.一种通过解离免疫复合物直接偶联制备免疫吸附剂的方法。
J Immunol Methods. 1980;32(4):327-38. doi: 10.1016/0022-1759(80)90025-3.
9
Comparison of antigen-type immunosorbents prepared by different ways.不同方法制备的抗原型免疫吸附剂的比较。
Ann Immunol Hung. 1975;18:85-92.
10
Immunoprotective murine monoclonal antibodies specific for the outer-core polysaccharide and for the O-antigen of Escherichia coli 0111:B4 lipopolysaccharide (LPS).对大肠杆菌O111:B4脂多糖（LPS）的外核心多糖和O抗原具有特异性的免疫保护性鼠单克隆抗体。
J Immunol. 1987 Jul 15;139(2):557-61.

引用本文的文献

1
Binding of mannose-binding protein to Klebsiella O3 lipopolysaccharide possessing the mannose homopolysaccharide as the O-specific polysaccharide and its relation to complement activation.甘露糖结合蛋白与以甘露糖同多糖作为O-特异性多糖的肺炎克雷伯菌O3脂多糖的结合及其与补体激活的关系。
Infect Immun. 1995 Jul;63(7):2537-40. doi: 10.1128/iai.63.7.2537-2540.1995.
2
Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.牛抗布鲁氏菌流产亚种脂多糖抗体的亲和纯化。
J Clin Microbiol. 1983 Feb;17(2):323-6. doi: 10.1128/jcm.17.2.323-326.1983.
3
Immobilization of microorganisms for detection by solid-phase immunoassays.用于固相免疫测定检测的微生物固定化。
J Clin Microbiol. 1985 Sep;22(3):361-5. doi: 10.1128/jcm.22.3.361-365.1985.
4
Induction of antitumor activities in spleen cells from mice and rats activated with lipopolysaccharide immobilized on beads.固定在珠子上的脂多糖激活的小鼠和大鼠脾细胞中抗肿瘤活性的诱导。
Cancer Immunol Immunother. 1992;35(1):39-45. doi: 10.1007/BF01741053.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Studies of enterobacterial lipopolysaccharides; effects of heat and chemicals on erythrocyte-modifying, antigenic, toxic and pyrogenic properties.肠杆菌脂多糖的研究；热和化学物质对红细胞修饰、抗原性、毒性及致热特性的影响。
J Immunol. 1956 May;76(5):377-85.
3
A colorimetric estimation of lipopolysaccharides.脂多糖的比色测定。
FEBS Lett. 1971 Sep 1;16(4):343-345. doi: 10.1016/0014-5793(71)80386-1.
4
Physical, chemical, and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate.由乙二胺四乙酸从大肠杆菌释放的脂多糖的物理、化学和免疫学特性。
J Biol Chem. 1968 Dec 25;243(24):6384-91.
5
Latex test for quantitative determination of Escherichia coli antibody.用于定量测定大肠杆菌抗体的乳胶试验。
Appl Microbiol. 1974 Dec;28(6):1073-5. doi: 10.1128/am.28.6.1073-1075.1974.
6
Conditions that affect the colorimetric analysis of lipopolysaccharide from Escherichia coli and Treponema pallidum.影响大肠杆菌和梅毒螺旋体中脂多糖比色分析的条件。
Appl Microbiol. 1973 Aug;26(2):129-33. doi: 10.1128/am.26.2.129-133.1973.
7
Detection of Escherichia coli antigens by a latex agglutination test.通过乳胶凝集试验检测大肠杆菌抗原。
Appl Microbiol. 1974 Aug;28(2):306-11. doi: 10.1128/am.28.2.306-311.1974.
8
Antigen distribution in a latex suspension and its relationship to test sensitivity.抗原在乳胶悬液中的分布及其与检测灵敏度的关系。
J Clin Microbiol. 1976 Jul;4(1):82-6. doi: 10.1128/jcm.4.1.82-86.1976.

大肠杆菌脂多糖与环氧活化的琼脂糖凝胶6B的偶联

Coupling of Escherichia coli lipopolysaccharide to epoxy-activated Sepharose 6B.

作者信息

Fox J, Hechemy K

出版信息

Infect Immun. 1978 Jun;20(3):867-8. doi: 10.1128/iai.20.3.867-868.1978.

DOI:10.1128/iai.20.3.867-868.1978
PMID:352965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC421940/
Abstract

Escherichia coli O111:B4 lipopolysaccharide was coupled to epoxy-activated Sepharose 6B. The bound lipopolysaccharide was immunogenic and immunoadsorptive although at less efficiency than free lipopolysaccharide.

摘要

将大肠杆菌O111:B4脂多糖偶联到环氧活化的琼脂糖凝胶6B上。结合的脂多糖具有免疫原性和免疫吸附性，尽管其效率低于游离脂多糖。