Muirhead D Y, Mallmann V H
Infect Immun. 1974 Aug;10(2):352-5. doi: 10.1128/iai.10.2.352-355.1974.
The effect of culture fluids from mouse lymphocytes on the antilisterial activity of normal macrophages in vitro was determined. Titers of lysates of macrophages incubated with or without culture fluids from lymphocytes from control mice had titers of 10 to 270 with or without a reducing agent, dithiothreitol. Lysates of macrophages after incubation with culture fluids of spleen lymphocytes from BCG-immunized mice had antilisterial titers with and without dithiothreitol of 197,000 and 810, respectively. This may indicate a dithiothreitol-dependent antilisterial system inherent in activated macrophages of cell-mediated immunity. Lysates of incubated spleen cells and their culture fluids also had slight antilisterial activity.
测定了小鼠淋巴细胞培养液对体外正常巨噬细胞抗李斯特菌活性的影响。与来自对照小鼠的淋巴细胞培养液一起孵育或未孵育的巨噬细胞裂解物，在有或没有还原剂二硫苏糖醇的情况下，效价为10至270。与卡介苗免疫小鼠的脾淋巴细胞培养液一起孵育后的巨噬细胞裂解物，在有和没有二硫苏糖醇的情况下，抗李斯特菌效价分别为197,000和810。这可能表明细胞介导免疫的活化巨噬细胞中存在一种依赖二硫苏糖醇的抗李斯特菌系统。孵育的脾细胞及其培养液的裂解物也有轻微的抗李斯特菌活性。
