Yasbin R E, Young F E
J Virol. 1974 Dec;14(6):1343-8. doi: 10.1128/JVI.14.6.1343-1348.1974.
Lysates of the virulent bacteriophage SPP1 were shown to be capable of mediating generalized transduction. Suppressible mutants of this bacteriophage (sus) were capable of transduction at a lower multiplicity of infection than virulent SPP1. Linkage analysis demonstrated that bacteriophage SPP1 transduced segments of the genome equal in size to that transferred by SP10. This bacteriophage should be useful in analyzing the regions of the genome where PBS1 appears to give anomalous results.
烈性噬菌体SPP1的裂解物被证明能够介导普遍性转导。这种噬菌体的可抑制突变体(sus)在感染复数较低时能够进行转导,而烈性噬菌体SPP1则需要较高的感染复数。连锁分析表明,噬菌体SPP1转导的基因组片段大小与SP10转移的片段相等。这种噬菌体在分析PBS1似乎给出异常结果的基因组区域时应该会很有用。