Halegoua S, Hirashima A, Inouye M
J Bacteriol. 1974 Dec;120(3):1204-8. doi: 10.1128/jb.120.3.1204-1208.1974.
The existence of a free form of a specific lipoprotein of molecular weight 7,200 was examined in the envelopes of several gram-negative bacteria. When the envelope proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, distinct peaks were observed in Salmonella typhimurium, Serratia marcescens, and Pseudomonas aeruginosa at the same position as the free form of the lipoprotein of Escherichia coli. However, the peak was not observed in Proteus mirabilis. The protein at the peak in S. typhimurium was shown to contain little or no histidine as expected from the amino acid composition of the lipoprotein. Furthermore, antiserum against the highly purified lipoprotein from E. coli was shown to react with the proteins from S. typhimurium and S. marcescens and to form the specific immunoprecipitates. In contrast, the protein from P. aeruginosa did not react with the antiserum at all. Thus, it is concluded that S. typhimurium and S. marcescens have the free form of the lipoprotein in their envelopes as does E. coli. P. aeruginosa contains a protein of the same size as the lipoprotein, but it is not certain whether the protein is the same structural protein as the lipoprotein from E. coli. P. mirabilis may not have any free form of the lipoprotein, may have it in a very small amount, or may have a lipoprotein of different molecular weight serving the same function.
在几种革兰氏阴性菌的包膜中检测了分子量为7200的特定脂蛋白游离形式的存在情况。当通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析包膜蛋白时，在鼠伤寒沙门氏菌、粘质沙雷氏菌和铜绿假单胞菌中观察到了与大肠杆菌脂蛋白游离形式处于相同位置的明显峰。然而，在奇异变形杆菌中未观察到该峰。鼠伤寒沙门氏菌中该峰处的蛋白质经显示，从脂蛋白的氨基酸组成来看，含有很少或不含组氨酸。此外，针对来自大肠杆菌的高度纯化脂蛋白的抗血清经显示可与鼠伤寒沙门氏菌和粘质沙雷氏菌的蛋白质发生反应，并形成特异性免疫沉淀。相比之下，铜绿假单胞菌的蛋白质根本不与抗血清发生反应。因此，可以得出结论，鼠伤寒沙门氏菌和粘质沙雷氏菌在其包膜中具有与大肠杆菌一样的脂蛋白游离形式。铜绿假单胞菌含有一种与脂蛋白大小相同的蛋白质，但尚不确定该蛋白质是否与来自大肠杆菌的脂蛋白为相同的结构蛋白。奇异变形杆菌可能没有任何脂蛋白游离形式，可能只有极少量，或者可能有一种分子量不同但功能相同的脂蛋白。
