使用冻融精子对人淋巴细胞进行HLA - D分型
HLA-D typing of human lymphocytes using frozen and thawed spermatozoa.
作者信息
Halim K, Festenstein H, Farrant J
出版信息
J Immunol Methods. 1979;25(1):31-6. doi: 10.1016/0022-1759(79)90161-3.
PMID:422849
Abstract
A method is described for the preservation of haploid populations of human spermatozoa in liquid nitrogen for their subsequent use as stimulators in HLA-D (SL) lymphocyte typing. The typing results obtained using thawed spermatozoa correlated well with those obtained when using fresh sperm and also with other cellular typing methods.
摘要
本文描述了一种将人类精子单倍体群体保存在液氮中，以便随后用作HLA - D（SL）淋巴细胞分型刺激物的方法。使用解冻精子获得的分型结果与使用新鲜精子获得的结果以及其他细胞分型方法获得的结果相关性良好。
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