Goldstein J, Roberts J W
J Clin Microbiol. 1982 May;15(5):848-51. doi: 10.1128/jcm.15.5.848-851.1982.
Studies were performed to determine the sensitivity and specificity of a new microtube method for the detection of coagulase production by Staphylococcus aureus. Rabbit plasma containing EDTA was added to and lyophilized in API microtubes. Two standard coagulase plasmas containing EDTA were used in the conventional macrotube test and served as a basis for comparison. No false-positive or false-negative reactions were encountered with the microtube system. With this system, 53% of the coagulase-positive strains tested were detected within 1 h after inoculation, 82% were detected after 2 h, 97% were detected after 3 h, and 99% were detected after 4 h. With the first conventional method, 45, 81, 96, and 98% of the positive strains were detected in 1, 2, 3, and 4 h, respectively, whereas with the second conventional method, only 6% of the positive strains were detected in 1 h, 24% in 2 h, 66% in 3 h, and 81% in 4 h. With the microtube method, 5 of the 139 coagulase-producing strains studied reverted to negative between 5 and 24 h after inoculation, whereas 9 reverted with the more rapid conventional method, and no reversions occurred with the second conventional method. All reversions involved strains which caused gelation of plasma within 1 h after inoculation. The data obtained showed that 99% of the coagulase-positive strains tested could be detected within 4 h by the microtube method. In addition, the microtube method offers a more convenient and economical format for the performance of the coagulase tube test.
开展了多项研究，以确定一种用于检测金黄色葡萄球菌凝固酶产生的新型微量管方法的敏感性和特异性。将含有乙二胺四乙酸（EDTA）的兔血浆加入API微量管中并冻干。在传统的大试管试验中使用了两种含有EDTA的标准凝固酶血浆，并作为比较的基础。微量管系统未出现假阳性或假阴性反应。使用该系统，接种后1小时内检测到53%的测试凝固酶阳性菌株，2小时后检测到82%，3小时后检测到97%，4小时后检测到99%。使用第一种传统方法，分别在1、2、3和4小时内检测到45%、81%、96%和98%的阳性菌株，而使用第二种传统方法，1小时内仅检测到6%的阳性菌株，2小时内检测到24%，3小时内检测到66%，4小时内检测到81%。使用微量管方法，在研究的139株产生凝固酶的菌株中，有5株在接种后5至24小时之间恢复为阴性，而使用更快速的传统方法有9株恢复为阴性，第二种传统方法未出现恢复情况。所有恢复均涉及接种后1小时内导致血浆凝胶化的菌株。获得的数据表明，通过微量管方法在4小时内可检测到99%的测试凝固酶阳性菌株。此外，微量管方法为进行凝固酶试管试验提供了一种更方便、经济的形式。
