兔血浆和猪血浆在试管凝固酶试验中的比较。
Comparison of rabbit and pig plasma in the tube coagulase test.
作者信息
Yrios J W
出版信息
J Clin Microbiol. 1977 Feb;5(2):221-4. doi: 10.1128/jcm.5.2.221-224.1977.
Abstract
A total of 627 clinical isolates of Micrococcaceae were characterized accoridng to their ability to coagulate rabbit and pig plasma, produce thermostable nuclease, and anaerobically ferment glucose and mannitol. By using these characteristics, 416 of the isolates were classified as Staphylococcus aureus and 211 as non-S. aureus. All 416 strains produced a 3 to 4+ clot formation in heparinized pig plasma, whereas 415 isolates produced a similar reaction in citrated rabbit plasma. All of the S. aureus strains possessed a thermostable nuclease, whereas only four of the non-S. aureus isolates exhibited this characteristic. The results obtained using heparinized pig plasma were almost identical to those obtained with commercial rabbit plasma.
摘要
根据凝固兔血浆和猪血浆的能力、产生耐热核酸酶以及厌氧发酵葡萄糖和甘露醇的能力，对总共627株微球菌科临床分离株进行了鉴定。利用这些特征，416株分离株被归类为金黄色葡萄球菌，211株为非金黄色葡萄球菌。所有416株菌株在肝素化猪血浆中产生3至4+的凝块形成，而415株分离株在枸橼酸盐兔血浆中产生类似反应。所有金黄色葡萄球菌菌株都具有耐热核酸酶，而非金黄色葡萄球菌分离株中只有4株表现出这一特征。使用肝素化猪血浆获得的结果与使用市售兔血浆获得的结果几乎相同。
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