Fontana C, Cellini L, Dainelli B
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università Tor Vergata di Roma, Italy.
J Clin Microbiol. 1993 Aug;31(8):2105-9. doi: 10.1128/jcm.31.8.2105-2109.1993.
A new biovar of Staphylococcus aureus subsp. aureus was isolated from human clinical specimens and described on the basis of studies of 12 isolates that were compared with 11 standard reference strains. Both DNA hybridization experiments and numerical taxonomy analysis demonstrated that these strains were strictly related to S. aureus subsp. aureus; however, they were significantly different from the latter. The atypical strains belonging to the new biovar can be distinguished from typical S. aureus subsp. aureus strains by their alpha-chymotrypsin, alpha-glucosidase, beta-N-acetylglucosaminidase, lipase (C-14), and leucine arylamidase enzymatic activities and novobiocin resistance. Thus, the combination of alpha-glucosidase and beta-N-acetyl-glucosaminidase is more useful for distinguishing these S. aureus strains from the other, typical ones.
从人类临床标本中分离出了金黄色葡萄球菌亚种的一个新生物变种,并基于对12株分离株的研究进行了描述,这些分离株与11株标准参考菌株进行了比较。DNA杂交实验和数值分类分析均表明,这些菌株与金黄色葡萄球菌亚种密切相关;然而,它们与后者存在显著差异。属于新生物变种的非典型菌株可通过其α-糜蛋白酶、α-葡萄糖苷酶、β-N-乙酰葡糖胺酶、脂肪酶(C-14)和亮氨酸芳基酰胺酶的酶活性以及对新生霉素的耐药性与典型的金黄色葡萄球菌亚种菌株区分开来。因此,α-葡萄糖苷酶和β-N-乙酰葡糖胺酶的组合对于区分这些金黄色葡萄球菌菌株与其他典型菌株更有用。