Ji S, Chance B, Nishiki K, Smith T, Rich T
Am J Physiol. 1979 Mar;236(3):C144-56. doi: 10.1152/ajpcell.1979.236.3.C144.
Three-way light guides containing one or more strands of 25-micron or 80-micron diameter optical fibers in each channel have been constructed and used to measure the NADH fluorescence and UV reflectance from mitochondrial suspensions, the perfused, hemoglobin-free rat liver, and the perfused beating interventricular septum of the rabbit. The optical changes measured with these so-called micro-light guides, which have channels containing one or several strands of optical fibers less than 100 micron, are comparable in magnitude with those measured using much larger conventional light guides. The effect of light scattering on the fluorescence channel has been determined and an empirical equation for correcting the fluorescence channel for light scattering has been obtained for mitochondrial suspensions. A mathematical equation characterizing the optical behavior of a two-way micro-light guide has been derived and has been shown to account satisfactorily for reflectance and fluorescence measurements of a mat surface in air.
已构建了三通道光导，每个通道包含一股或多股直径为25微米或80微米的光纤，并用于测量线粒体悬浮液、灌注的无血红蛋白大鼠肝脏以及兔灌注搏动的室间隔中的NADH荧光和紫外线反射率。这些所谓的微光导，其通道包含一股或多股小于100微米的光纤，所测量的光学变化在幅度上与使用大得多的传统光导所测量的变化相当。已确定了光散射对荧光通道的影响，并获得了用于校正线粒体悬浮液光散射的荧光通道的经验方程。已推导了表征双向微光导光学行为的数学方程，并已证明该方程能令人满意地解释空气中粗糙表面的反射率和荧光测量结果。
