Barabas A Z, Lannigan R
Br J Exp Pathol. 1974 Jun;55(3):282-90.
The early effects of a single intravenous injection of anti-rat kidney tubular fraction 3 antibody were studied in rats pretreated with BSA and in untreated animals. Definite deposition of immune complexes, which stained for rat IgG, were noted in the glomeruli of the BSA pretreated rats after one day and in the untreated rats by the fourth day with the fluorescent antibody technique. Control animals injected with normal rabbit serum or BSA and normal controls did not develop immune complex glomerulonephritis. Two of the 4 surviving rats pretreated with BSA and injected with the anti-tubular fraction 3 antibody developed proteinuria towards the fourth week, whereas proteinuria did not occur in the untreated and the control rats. It is possible that the heterologous anti-rat kidney tubular fraction 3 antibody releases a nephritogenic antigen from the proximal convoluted tubules soon after its administration. Autoantibodies formed are presumably able to form immune complexes with the nephritogenic antigen and produce glomerular injury. As a result of the glomerular damage, the autoantibodies may gain access to the proximal convoluted tubular cells and maintain the release of the nephritogenic antigen. The developing kidney disease is morphologically similar in every respect to autologous immune complex glomerulonephritis.
在预先用牛血清白蛋白（BSA）处理的大鼠和未处理的动物中，研究了单次静脉注射抗大鼠肾小管3组分抗体的早期效应。采用荧光抗体技术，在预先用BSA处理的大鼠肾小球中，一天后就观察到了明确的免疫复合物沉积，这些复合物对大鼠IgG呈阳性染色；在未处理的大鼠中，到第四天时也观察到了这种沉积。注射正常兔血清或BSA的对照动物以及正常对照组均未发生免疫复合物性肾小球肾炎。4只预先用BSA处理并注射抗肾小管3组分抗体的存活大鼠中，有2只在第四周左右出现蛋白尿，而未处理的大鼠和对照大鼠未出现蛋白尿。有可能异源性抗大鼠肾小管3组分抗体在给药后不久就从近端曲管释放出一种致肾炎抗原。形成的自身抗体大概能够与致肾炎抗原形成免疫复合物并造成肾小球损伤。由于肾小球损伤，自身抗体可能进入近端曲管细胞并维持致肾炎抗原的释放。所发生的肾脏疾病在形态学上与自身免疫复合物性肾小球肾炎在各方面都相似。
