Maxie M G, Valli V E
Can J Comp Med. 1974 Oct;38(4):383-90.
(3)H-labelled Pseudomonas endotoxin was incubated in vitro with blood from nontolerant and endotoxin tolerant calves. Formed elements were separated from serial samples of the incubated mixtures. The labelled endotoxin became associated with neutrophils, monocytes, lymphocytes, platelets and erythrocytes. Association of (3)H-endotoxin with formed elements of the blood occurred during the first five minutes of incubation and did not significantly change over the course of a three hour incubation period. Tolerance did not result in increased uptake of (3)H-endotoxin by formed elements of the blood. Tolerance of calves to endotoxin is apparently not due to increased uptake of endotoxin by formed elements of the blood. The Limulus amebocyte lysate assay was unreliable for the detection of endotoxin which was present in calf blood in vitro and requires further modification.
（3）将H标记的假单胞菌内毒素与未耐受和内毒素耐受小牛的血液在体外进行孵育。从孵育混合物的系列样本中分离出有形成分。标记的内毒素与中性粒细胞、单核细胞、淋巴细胞、血小板和红细胞结合。在孵育的前五分钟内，（3）H-内毒素与血液有形成分发生结合，并且在三小时的孵育期内没有显著变化。耐受性并未导致血液有形成分对（3）H-内毒素的摄取增加。小牛对内毒素的耐受性显然不是由于血液有形成分对内毒素的摄取增加所致。鲎试剂检测法对于检测体外小牛血液中存在的内毒素不可靠，需要进一步改进。
