Roth R I, Levin F C, Levin J
Department of Laboratory Medicine, University of California School of Medicine, San Francisco.
Infect Immun. 1993 Aug;61(8):3209-15. doi: 10.1128/iai.61.8.3209-3215.1993.
Bacterial endotoxin (lipopolysaccharide [LPS]) is known to interact with numerous components of blood, including erythrocytes, mononuclear cells, platelets, neutrophils, lipoproteins, and plasma proteins. The relative affinities of LPS for these elements, and the distribution of LPS between them, are unknown. Cross-linked stroma-free hemoglobin (SFH), a potential substitute for erythrocyte transfusion, produces in vivo toxicity in animals consistent with significant LPS contamination. Therefore, we studied the distribution of LPS in human and rabbit blood and examined whether the presence of SFH altered LPS distribution. In either the presence or absence of SFH, LPS was associated predominantly with high-density lipoproteins and apoproteins. There was lesser binding to low- and very-low-density lipoproteins. Examination of the apoprotein pool by column chromatography and density centrifugation demonstrated that LPS in this fraction was predominantly protein bound. Binding of LPS to SFH resulted in dissociation of a portion of the LPS into low-molecular-weight complexes. Cell-bound LPS was only 2 to 16% of the total and was unaffected by SFH. The distribution among blood cells demonstrated predominant binding to platelets in human blood but predominant binding to erythrocytes in rabbit blood. Cellular distribution was not significantly altered by SFH.
已知细菌内毒素（脂多糖[LPS]）可与血液中的多种成分相互作用，包括红细胞、单核细胞、血小板、中性粒细胞、脂蛋白和血浆蛋白。LPS对这些成分的相对亲和力以及LPS在它们之间的分布尚不清楚。交联无基质血红蛋白（SFH）是一种潜在的红细胞输血替代品，在动物体内会产生与严重LPS污染一致的毒性。因此，我们研究了LPS在人和兔血液中的分布，并检查了SFH的存在是否会改变LPS的分布。无论有无SFH，LPS主要与高密度脂蛋白和载脂蛋白相关。与低密度和极低密度脂蛋白的结合较少。通过柱色谱和密度离心对载脂蛋白库进行检查表明，该部分中的LPS主要与蛋白质结合。LPS与SFH的结合导致一部分LPS解离成低分子量复合物。细胞结合的LPS仅占总量的2%至16%，且不受SFH影响。血细胞之间的分布表明，LPS在人血中主要与血小板结合，而在兔血中主要与红细胞结合。细胞分布未因SFH而发生显著改变。
