Tomasulo P A, Levin J, Murphy P A, Winkelstein J A
J Lab Clin Med. 1977 Feb;89(2):308-15.
Tritiated endotoxins were prepared by three different methods. The biological activities of the tritiated endotoxins were determined by the Limulus amebocyte lysate assay, a rabbit pyrogen assay, and a complement-activation assay and were compared to native, unlabeled endotoxin. All three tritiated endotoxin preparations manifested adequate biological activity in each of the three assay systems, and all three assays ranked the biological activity of the different endotoxin preparations in the same order. Endotoxin tritiated by the Wilzbach procedure retained most of its biological activity and also had the highest specific radioactivity. The good correlation between the Limulus lysate, rabbit pyrogen, and complement-activation assays suggests that the same active site of the endotoxin molecule is identified by the three different assays.
通过三种不同方法制备了氚标记的内毒素。采用鲎试剂法、家兔热原试验和补体激活试验测定了氚标记内毒素的生物活性，并与天然未标记内毒素进行了比较。所有三种氚标记内毒素制剂在三种检测系统中均表现出足够的生物活性，并且所有三种检测方法对不同内毒素制剂生物活性的排序相同。通过威尔兹巴赫法氚标记的内毒素保留了大部分生物活性，并且具有最高的比放射性。鲎试剂法、家兔热原试验和补体激活试验之间的良好相关性表明，三种不同的检测方法识别的是内毒素分子的同一活性位点。
