在不同细胞培养物中分离肠道病毒。
Enteric virus isolation in different cell cultures.
作者信息
Lee L H, Phillips C A, South M A, Melnick J L, Yow M D
出版信息
Bull World Health Organ. 1965;32(5):657-63.
The need for establishing an optimum tissue culture cell line for isolation of enteric viruses prompted a comparative study of cell lines for the isolation of enteric viruses from rectal swab specimens. The viruses isolated included all three types of poliovirus, 12 types of echovirus, seven types of group A and two of group B coxsackievirus, 24 strains of adenovirus, and eight untyped agents which probably represent new enteroviruses. One hundred positive isolations were made from 387 rectal swab specimens; 86 were obtained in human kidney cultures. This was almost twice the number obtained with monkey kidney and more than one-and-a-half times that obtained with human lung cells (WI-38). A diploid human epithelial cell line (Attleson) was tested and was found to be inferior to both monkey kidney and WI-38 cells for enteric viruses. Human kidney cells were particularly useful for group A coxsackieviruses and adenoviruses.
为了建立用于分离肠道病毒的最佳组织培养细胞系，促使人们对从直肠拭子标本中分离肠道病毒的细胞系进行了一项比较研究。分离出的病毒包括所有三种类型的脊髓灰质炎病毒、12种埃可病毒、7种A组和2种B组柯萨奇病毒、24株腺病毒，以及8种未分型病原体，这些病原体可能代表新型肠道病毒。从387份直肠拭子标本中进行了100次阳性分离；86次是在人肾培养物中获得的。这几乎是在猴肾培养物中获得数量的两倍，是在人肺细胞（WI-38）中获得数量的一倍半以上。对一种二倍体人上皮细胞系（阿特尔森）进行了测试，发现其在分离肠道病毒方面不如猴肾细胞和WI-38细胞。人肾细胞对A组柯萨奇病毒和腺病毒特别有用。
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