Canada J C, Strong D H
Appl Microbiol. 1965 Sep;13(5):788-92. doi: 10.1128/am.13.5.788-792.1965.
The resistance to heat, as measured by D values and phantom thermal death time curves, was observed to increase for one of three strains of Clostridium perfringens type A subsequent to animal passage. Animal passage was accomplished by the force-feeding of germ-free mice with bacterial suspensions of the organism, followed by the force-feeding of additional gnotobiotic mice with the contaminated feces. For the one strain in which an increase in heat resistance was noted, the result could not be attributed to mouse feces per se, since the presence of sterile germ-free mouse feces in a suspending medium did not protect C. perfringens spores from elevated temperature destruction.
通过D值和热死时间曲线测量，观察到产气荚膜梭菌A型三种菌株中的一种在动物传代后对热的抗性增加。动物传代是通过向无菌小鼠强制喂食该生物体的细菌悬液，然后再向其他无菌动物小鼠强制喂食受污染的粪便来完成的。对于观察到耐热性增加的那一种菌株，该结果不能归因于小鼠粪便本身，因为悬浮培养基中无菌无菌小鼠粪便的存在并不能保护产气荚膜梭菌孢子免受高温破坏。
