Kim K, Groman N B
J Bacteriol. 1965 Dec;90(6):1552-6. doi: 10.1128/jb.90.6.1552-1556.1965.
Kim, K. (University of Washington, Seattle), and N. B. Groman. In vitro inhibition of diphtheria toxin action by ammonium salts and amines. J. Bacteriol. 90:1552-1556. 1965.-An inhibitor for diphtheria toxin action on HeLa cells was demonstrated in the growth supernatant fractions of both toxinogenic and nontoxinogenic strains of Corynebacterium diphtheriae and in the Mueller and Miller medium in which these organisms were grown. The inhibitor in the growth supernatant fractions of the nontoxinogenic strain was dialyzable, stable to autoclaving, and stable on storage in the refrigerator for a period of many months, but was destroyed by ashing. When the components of Mueller and Miller medium were analyzed, only the Casamino Acids proved inhibitory. Further study with artificial mixtures of amino acids revealed that glutamine alone inhibited toxin. It was subsequently shown that ammonium salts and the aliphatic amines, glycamine and prolamine, could also function as inhibitors. Histamine and 16 amino acids tested individually were ineffective. The effectiveness of the amines and the ineffectiveness of sodium or potassium ions indicates that there is a specific requirement for inhibition.
金，K.（华盛顿大学，西雅图）和N.B.格罗曼。铵盐和胺对体外白喉毒素作用的抑制。《细菌学杂志》90:1552 - 1556。1965年。——在产毒素和不产毒素的白喉棒状杆菌菌株的生长上清液组分以及这些菌株生长所用的米勒培养基中，均证实存在一种对白喉毒素作用于海拉细胞的抑制剂。不产毒素菌株生长上清液组分中的抑制剂可透析，对高压灭菌稳定，在冰箱中储存数月也稳定，但经灰化会被破坏。对米勒培养基的成分进行分析时，只有酪蛋白氨基酸被证明具有抑制作用。对氨基酸人工混合物的进一步研究表明，仅谷氨酰胺能抑制毒素。随后发现，铵盐以及脂肪族胺、甘胺和谷胺也可作为抑制剂。单独测试的组胺和16种氨基酸均无作用。胺的有效性以及钠离子或钾离子的无效性表明，抑制存在特定要求。
