Lindenmann J, Klein P A
J Exp Med. 1967 Jul 1;126(1):93-108. doi: 10.1084/jem.126.1.93.
A2G mice could be solidly immunized against the Ehrlich ascites tumor by single intraperitoneal injections of homogenized and lyophilized tumor cells which had been infected with oncolytic strains of influenza A virus. Similar homogenates from noninfected tumor cells were not immunogenic, even when mixed with egg-grown virus. The immunizing principle in viral oncolysates could not be separated from the oncolytic virus by differential centrifugation or adsorption to and elution from red cells. It could be inhibited by antibody raised in rabbits against the egg-grown oncolytic virus. This reaction showed serologic specificity. Thus, the immunogenicity of an oncolysate produced with the WSA strain of neurotropic influenza virus could be inhibited by rabbit anti-WSA, but not by rabbit antibody to the TUR strain of fowl plague virus. Conversely, the immunogenicity of an oncolysate prepared with the TUR strain could be inhibited by rabbit anti-TUR, but not by anti-WSA. When mice were preimmunized (primed) with egg-grown WSA virus, their antitumor response to a later injection of WSA oncolysate was of the anamnestic type. Priming with egg-grown influenza B virus had no such effect. It was concluded that the immunogenicity of certain host cell components was greatly increased by incorporation into the makeup of the oncolytic virus.
通过单次腹腔注射经甲型流感病毒溶瘤株感染的匀浆冻干肿瘤细胞，A2G小鼠能够有效免疫抵御艾氏腹水瘤。即使与鸡胚培养的病毒混合，未感染肿瘤细胞的类似匀浆也没有免疫原性。病毒溶瘤产物中的免疫原性成分无法通过差速离心或与红细胞吸附及洗脱来与溶瘤病毒分离。它可被兔抗鸡胚培养的溶瘤病毒产生的抗体所抑制。这种反应具有血清学特异性。因此，嗜神经性流感病毒WSA株产生的溶瘤产物的免疫原性可被兔抗WSA抑制，但不能被鸡瘟病毒TUR株的兔抗体抑制。相反，TUR株制备的溶瘤产物的免疫原性可被兔抗TUR抑制，但不能被抗WSA抑制。当小鼠用鸡胚培养的WSA病毒进行预免疫（致敏）时，它们对随后注射的WSA溶瘤产物的抗肿瘤反应属于回忆反应类型。用鸡胚培养的乙型流感病毒致敏则没有这种效果。得出的结论是，某些宿主细胞成分的免疫原性通过整合到溶瘤病毒的组成中而大大增强。
