Mickelson M N
J Bacteriol. 1967 Jul;94(1):184-91. doi: 10.1128/jb.94.1.184-191.1967.
Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically grown cells, as percentage of glucose carbon, were 52 to 61% lactic acid, 20 to 23% acetic acid, 5.5 to 6.5% acetylmethylcarbinol, and 14 to 16% carbon dioxide. There was no evidence for a pentose cycle or a tricarboxylic acid cycle. Crude cell-free extracts of S. agalactiae 50 possessed a strong reduced nicotinamide adenine dinucleotide (NADH(2)) oxidase that is also cyanide-sensitive. Dialysis or ultrafiltration of the crude, cell-free extract resulted in loss of NADH(2) oxidase activity. Oxidase activity was restored to the inactive extract by addition of the ultrafiltrate or by addition of menadione or K(3)Fe(CN)(6). Noncytochrome iron-containing pigments were present in cell-free extracts of S. agalactiae. The possible participation of these pigments in the respiration of S. agalactiae is presently being studied.
无乳链球菌培养物拥有一条用于葡萄糖氧化的需氧途径，该途径受到氰化物的强烈抑制。无乳链球菌50株需氧生长细胞氧化葡萄糖的产物是乳酸、乙酸、乙酰甲基甲醇和二氧化碳。需氧生长细胞产生的葡萄糖降解产物，以葡萄糖碳的百分比计，乳酸为52%至61%，乙酸为20%至23%，乙酰甲基甲醇为5.5%至6.5%，二氧化碳为14%至16%。没有证据表明存在戊糖循环或三羧酸循环。无乳链球菌50株的粗制无细胞提取物具有一种强还原型烟酰胺腺嘌呤二核苷酸（NADH₂）氧化酶，该酶也对氰化物敏感。对粗制无细胞提取物进行透析或超滤会导致NADH₂氧化酶活性丧失。通过添加超滤物或添加甲萘醌或铁氰化钾（K₃Fe(CN)₆）可使无活性提取物恢复氧化酶活性。无乳链球菌的无细胞提取物中存在非细胞色素含铁色素。目前正在研究这些色素在无乳链球菌呼吸作用中的可能参与情况。
