一种用于估算脂质中极少量氮的简单方法。
A simple procedure for the estimation of very small amounts of nitrogen in lipids.
作者信息
Sloane-Stanley G H
出版信息
Biochem J. 1967 Jul;104(1):293-5. doi: 10.1042/bj1040293.
Abstract
- About 0.1mug.atom of combined nitrogen, in lipids and a few other compounds, can be determined quantitatively by the gentle digestion of dry samples in 10ml. test tubes with perchloric acid for 30min., followed by the estimation of the resulting ammonia as the stable blue colour (lambda(max.) 635mmu) produced by the addition of phenol and nitroprusside, an alkaline phosphate buffer and alkaline hypochlorite. 2. Deionized distilled water is required, but the other reagents need no special purification if chosen and handled with care. 3. The results are linear with from 0.015 to 0.15mug.atom of nitrogen, or up to 1mug.atom if the final solutions are diluted with water after full colour development.
摘要
- 脂质及其他一些化合物中约0.1微克原子的结合态氮，可通过在10毫升试管中用高氯酸对干燥样品进行温和消化30分钟来定量测定，随后通过加入苯酚和硝普钠、碱性磷酸盐缓冲液及碱性次氯酸盐后产生的稳定蓝色（最大吸收波长635纳米）来估算生成的氨。2. 需要去离子蒸馏水，但如果小心选择和处理，其他试剂无需特殊纯化。3. 结果在0.015至0.15微克原子氮范围内呈线性，若在显色完全后用水稀释最终溶液，则可测定高达1微克原子氮。
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