Parks W P, Casazza A M, Alcott J, Melnick J L
J Exp Med. 1968 Jan 1;127(1):91-108. doi: 10.1084/jem.127.1.91.
Adeno-associated satellite virus type 4 interferes with the replication of its helper adenovirus. No interferon-like soluble substance could be detected in satellite-infected cultures and other DNA- and RNA-containing viruses were not inhibited by coinfection with satellite virus under conditions which reduced adenovirus yields by more than 90% in monkey cells. Altering the concentration of adenovirus in the presence of constant amounts of satellite resulted in a constant degree of interference over a wide range of adenovirus inocula and suggested that adenovirus concentration was not a significant factor in the observed interference. The interference with adenovirus replication was abolished by pretreating satellite preparations with specific antiserum, ultraviolet light or heating at 80 degrees C for 30 min. This suggested that infectious satellite virus mediated the interference. Satellite virus concentration was found to be a determinant of interference and studies indicated that the amount of interference with adenovirus was directly proportional to the concentration of satellite virus. 8 hr after adenovirus infection, the replication of adenovirus was no longer sensitive to satellite interference. This was true even though the satellite virus was enhanced as effectively as if the cells were infected simultaneously with both viruses. Interference with adenovirus infectivity was accompanied by reduced yields of complement-fixing antigen and of virus particles which suggested that satellite virus interfered with the formation and not the function of adenovirus products. When cells were infected either with adenovirus alone or with adenovirus plus satellite, the same proportion of cells plated as adenovirus infectious centers. However, the number of plaque-forming units of adenovirus formed per cell in the satellite-infected cultures was reduced by approximately 90%, the same magnitude of reduction noted in whole cultures coinfected with satellite and adenovirus. This suggested that all cells infected with the two viruses were producing a reduced quantity of adenovirus.
4型腺相关卫星病毒会干扰其辅助腺病毒的复制。在卫星病毒感染的培养物中未检测到类似干扰素的可溶性物质，并且在猴细胞中能使腺病毒产量降低90%以上的条件下，其他含DNA和RNA的病毒不会因与卫星病毒共感染而受到抑制。在卫星病毒量恒定的情况下改变腺病毒浓度，在广泛的腺病毒接种范围内产生恒定程度的干扰，这表明腺病毒浓度并非观察到的干扰的重要因素。用特异性抗血清、紫外线或在80℃加热30分钟预处理卫星制剂，可消除对腺病毒复制的干扰。这表明感染性卫星病毒介导了干扰。发现卫星病毒浓度是干扰的决定因素，研究表明对腺病毒的干扰量与卫星病毒浓度成正比。腺病毒感染8小时后，其复制对卫星干扰不再敏感。即便卫星病毒得到了与细胞同时感染两种病毒时同样有效的增强，情况依然如此。对腺病毒感染性的干扰伴随着补体结合抗原和病毒颗粒产量的降低，这表明卫星病毒干扰的是腺病毒产物的形成而非功能。当细胞单独感染腺病毒或同时感染腺病毒和卫星病毒时，接种的细胞作为腺病毒感染中心的比例相同。然而，在卫星病毒感染的培养物中，每个细胞形成的腺病毒空斑形成单位数量减少了约90%，这与卫星病毒和腺病毒共感染的整个培养物中观察到的减少幅度相同。这表明所有感染这两种病毒的细胞产生的腺病毒量都减少了。
