Marples R R, Towers A G
J Hyg (Lond). 1979 Apr;82(2):237-48. doi: 10.1017/s0022172400025651.
The model was based on grasping a fabric-covered bottle contaminated with a strain of Staphylococcus saprophyticus, then grasping a sterile fabric-covered bottle and counting the organisms transferred. When the donor fabric was moist 10% of the available cells passed onto the hands, but this fell to 0.05% when the inoculum dried. Transfer from wet hands to the fabric amounted to 85%, but in the complete model only 0.06% of the available cells were transferred. The model was used to assess simple methods of degerming the hands. Washing the hands reduced transfer by 95%, while washing in 70% alcohol reduced transfer by 99.99%. Lesser procedures investigated included applying 0.2 ml of 80% ethanol to the hands, which reduced transfer by 93%.
该模型基于先抓取一个被腐生葡萄球菌菌株污染的织物包裹瓶，然后抓取一个无菌的织物包裹瓶并计算转移的微生物数量。当供体织物潮湿时，10%的可用细胞会转移到手上，但接种物干燥后这一比例降至0.05%。从湿手转移到织物上的比例为85%，但在完整模型中，只有0.06%的可用细胞被转移。该模型用于评估手部除菌的简单方法。洗手可使转移减少95%，而用70%酒精洗手可使转移减少99.99%。研究的其他较少的操作包括将0.2毫升80%乙醇涂在手上，这可使转移减少93%。
