Lundgren E, Larsson I, Miörner H, Strannegård O
J Gen Virol. 1979 Mar;42(3):589-95. doi: 10.1099/0022-1317-42-3-589.
Serum-depleted human foetal skin fibroblasts were stimulated by addition of 10% foetal calf serum to proliferate synchronously for at least one cell cycle. This proliferation was suppressed by leukocyte or fibroblast interferon (IF), which prolonged the G1 phase and diminished the rate of DNA synthesis during the S phase in a dose-dependent manner. When used in identical concentration, as judged in terms of units of antiviral activity, fibroblast IF had more pronounced effects on cell cycle events than leukocyte IF. Interferon exerted its effect in early G1, before the cells were irreversibly committed to DNA synthesis.
通过添加10%胎牛血清刺激去除血清的人胎儿皮肤成纤维细胞，使其同步增殖至少一个细胞周期。白细胞或成纤维细胞干扰素（IF）可抑制这种增殖，其以剂量依赖的方式延长G1期并降低S期DNA合成速率。当以抗病毒活性单位判断使用相同浓度时，成纤维细胞IF对细胞周期事件的影响比白细胞IF更显著。干扰素在G1早期发挥作用，此时细胞尚未不可逆地进入DNA合成阶段。
