Sturman L S, Tamm I
J Virol. 1969 Jan;3(1):8-16. doi: 10.1128/JVI.3.1.8-16.1969.
GDVII virus growth in BHK-21 cells, a permissive host for the virus, resembled productive infections with other picornaviruses. Virus yields ranged from 100 to 600 plaque-forming units (PFU)/cell. Virus replication in HeLa cells, a nonpermissive host for GDVII virus, was characterized by virus yields of only 0.1 to 5 PFU/cell. Similar low yields of virus have been obtained from HeLa cells at all multiplicities of input up to 6,000 per cell. The progeny particles from HeLa cells were, like the infecting particles, restricted in the HeLa cell host. Despite the great difference in final yields of virus from BHK-21 and HeLa cells, the times when maximal yields were reached were similar. GDVII virus stock grown in BHK-21 cells was designated HeLa(-). A variant of GDVII virus which is capable of extensive growth in HeLa cells was obtained. This variant, designated HeLa(+) GDVII virus, was passaged serially in HeLa cells. Virus yields of 50 to 150 infective virus particles per cell were obtained from infection of HeLa cells with HeLa(+) GDVII virus. The major species of HeLa(+) virus-specific ribonucleic acid (RNA) produced was single stranded and sedimented with an S value of 35S. The rate of accumulation of HeLa(+) virus-specific RNA in HeLa cell cultures was about four times that of HeLa(-) RNA. The amount of virus-specific HeLa(+) RNA formed in HeLa cells was several-fold greater than that of HeLa(-) RNA. With HeLa(-) parent GDVII virus undergoing productive replication in BHK-21 cells or abortive replication in HeLa cells, the major species of virus-specific RNA produced was single stranded and sedimented with an approximate S value of 35S. The amount of HeLa(-) virus-specific RNA extracted from BHK-21 cells was several-fold greater than the amount obtained from HeLa cells.
GDVII病毒在BHK - 21细胞（该病毒的允许性宿主）中的生长情况与其他小RNA病毒的增殖性感染相似。病毒产量为每细胞100至600个空斑形成单位（PFU）。GDVII病毒在HeLa细胞（该病毒的非允许性宿主）中的复制特点是病毒产量仅为每细胞0.1至5个PFU。在每细胞高达6000个的所有输入复数下，HeLa细胞都获得了类似的低病毒产量。来自HeLa细胞的子代颗粒，如同感染颗粒一样，在HeLa细胞宿主中受到限制。尽管BHK - 21细胞和HeLa细胞最终的病毒产量差异很大，但达到最大产量的时间相似。在BHK - 21细胞中培养的GDVII病毒株被命名为HeLa(-)。获得了一种能够在HeLa细胞中大量生长的GDVII病毒变体。这种变体被命名为HeLa(+) GDVII病毒，并在HeLa细胞中连续传代。用HeLa(+) GDVII病毒感染HeLa细胞可获得每细胞50至150个感染性病毒颗粒的产量。产生的HeLa(+)病毒特异性核糖核酸（RNA）的主要种类是单链的，沉降系数为35S。HeLa(+)病毒特异性RNA在HeLa细胞培养物中的积累速率约为HeLa(-) RNA的四倍。HeLa细胞中形成的病毒特异性HeLa(+) RNA的量比HeLa(-) RNA大几倍。对于在BHK - 21细胞中进行增殖性复制或在HeLa细胞中进行流产性复制的HeLa(-)亲本GDVII病毒，产生的病毒特异性RNA的主要种类是单链的，沉降系数约为35S。从BHK - 21细胞中提取的HeLa(-)病毒特异性RNA的量比从HeLa细胞中获得的量高几倍。
