Sidwell R W, Dixon G J, Westbrook L, Forziati F H
Appl Microbiol. 1971 Feb;21(2):227-34. doi: 10.1128/am.21.2.227-234.1971.
The effects of laundering with both anionic and nonionic detergents in cold, warm, and hot water on poliovirus-contaminated cotton sheeting, cotton terry cloth, washable wool shirting, wool blanketing, dull nylon jersey, and dacron/cotton shirting were determined. The fabrics were exposed to virus by aerosolization and direct contact (pipette) in separate studies. Although the results varied with each factor used in the study, virus titers on all the fabrics were generally reduced considerably by the laundering process. When the fabrics were dried for 20 hr after laundering, an additional decline in virus titers was seen, often to below detectable levels. The type of detergent used made little difference in effect on virus titer reduction, but the hot wash water markedly reduced the detectable virus. Fabric type was not a major factor in the majority of the experiments, although virus tended to be eliminated more readily from the nylon jersey, and in warm water the virus persisted longer on wool blanketing material laundered in anionic detergent. Sterile fabrics of each type laundered with similar fabrics which contained virus often became contaminated by the virus during the laundering process. Virus titers ranging from undetectable to 10(3.9) cell culture 50% infectious doses/ml were obtained from samples of the rinse water after warm- and cold-water laundering.
研究了在冷、温、热水中使用阴离子和非离子洗涤剂洗涤对受脊髓灰质炎病毒污染的棉质床单、棉毛巾布、可水洗羊毛衬衫布、羊毛毯子、无光尼龙针织品以及涤纶/棉衬衫布的影响。在单独的研究中，通过雾化和气溶胶化以及直接接触（移液管）使织物接触病毒。尽管研究中使用的每个因素的结果各不相同，但通过洗涤过程，所有织物上的病毒滴度通常都大幅降低。当织物在洗涤后干燥20小时时，病毒滴度进一步下降，通常降至检测水平以下。所用洗涤剂的类型对病毒滴度降低的效果影响不大，但热水洗涤显著降低了可检测到的病毒。在大多数实验中，织物类型不是主要因素，尽管尼龙针织品中的病毒往往更容易被清除，并且在温水中，用阴离子洗涤剂洗涤的羊毛毯子材料上的病毒持续时间更长。用含有病毒的类似织物洗涤的每种类型的无菌织物在洗涤过程中常常会被病毒污染。从温水和冷水洗涤后的漂洗水样品中获得的病毒滴度范围为不可检测到10(3.9)细胞培养50%感染剂量/毫升。
