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关于织物作为病毒传播媒介的定量研究。IV. 织物干接触传播病毒

Quantitative studies on fabrics as disseminators of viruses. IV. Virus transmission by dry contact of fabrics.

作者信息

Sidwell R W, Dixon G J, Westbrook L, Forziati F H

出版信息

Appl Microbiol. 1970 Jun;19(6):950-4. doi: 10.1128/am.19.6.950-954.1970.

DOI:10.1128/am.19.6.950-954.1970
PMID:4318451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376831/
Abstract

Cotton and woolen fabrics and fabrics of synthetic fibers were exposed by direct contact (pipette) and by aerosolization to poliovirus and to vaccinia virus in separate experiments, allowed to dry for 16 hr at 25 C in 35% relative humidity, and randomly tumbled with sterile swatches of the same fabrics for 30 min. By use of a HEp-2 cell assay system, up to 10(3.5) CCID(50) of poliovirus per ml and 10(4.4) CCID(50) of vaccinia virus per ml were recovered from the originally sterile fabrics as early as 1 to 10 min after contact. Maximum transfer of both viruses was achieved with wool blanket material, although high titers of vaccinia virus were recovered from all fabrics tested. Poliovirus placed on the fabrics in an aerosol tended to be transferred to the sterile fabrics at a greater rate than when it was placed on the fabrics by direct contact. The method of exposure had essentially no effect on the rate of transfer of vaccinia virus.

摘要

在单独的实验中,通过直接接触(移液管)和气雾化的方式,将棉织物、羊毛织物及合成纤维织物暴露于脊髓灰质炎病毒和痘苗病毒中,在25℃、相对湿度35%的条件下干燥16小时,然后与相同织物的无菌样本随机翻滚30分钟。使用HEp-2细胞检测系统,在接触后最早1至10分钟,每毫升从原本无菌的织物中就可回收高达10(3.5) CCID(50)的脊髓灰质炎病毒和10(4.4) CCID(50)的痘苗病毒。虽然从所有测试织物中都回收了高滴度的痘苗病毒,但羊毛毯材料实现了两种病毒的最大转移。以气溶胶形式置于织物上的脊髓灰质炎病毒比通过直接接触置于织物上时,往往以更高的速率转移至无菌织物上。暴露方式对痘苗病毒的转移速率基本没有影响。

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Potentially infectious agents associated with shearling bedpads. I. Effect of laundering with detergent-disinfectant combinations on polio and vaccinia viruses.与剪羊毛褥垫相关的潜在感染因子。I. 用洗涤剂 - 消毒剂组合洗涤对脊髓灰质炎病毒和痘苗病毒的影响。
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