Duncan C L, Sugiyama H, Strong D H
J Bacteriol. 1968 May;95(5):1560-6. doi: 10.1128/jb.95.5.1560-1566.1968.
The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C in Skim Milk (Difco), 14 of 29 type A strains isolated from food-poisoning outbreaks consistently produced exudation of fluid and consequent dilation of the ileal segments. In contrast, 15 of the 18 strains derived from other sources failed to elicit a response. By use of different inoculum preparations, nearly all strains could be made to give at least an occasional positive loop reaction. Diarrhea was not obtained in rabbits by intraluminal injection into the normal ileum or by per os administration of the cultures. Lecithinase, purified and in concentrated culture supernatant fractions, failed to produce a response in the isolated ileal loops.
对兔肠结扎环进行了研究，作为产气荚膜梭菌食物中毒研究的一种可能的实验模型。制备攻击接种物的方法对于确定给定菌株是否会引发反应很重要。当培养物在脱脂乳（Difco）中于37℃培养4小时时，从食物中毒暴发中分离出的29株A型菌株中有14株始终产生液体渗出并导致回肠段扩张。相比之下，来自其他来源的18株菌株中有15株未能引发反应。通过使用不同的接种物制备方法，几乎所有菌株都可以至少偶尔产生阳性环反应。通过向正常回肠腔内注射或经口给予培养物，未在兔中获得腹泻。纯化的和浓缩培养上清液组分中的卵磷脂酶在分离的回肠环中未能产生反应。
