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预湿对选定空气传播病毒采样的影响。

Effect of prehumidification on sampling of selected airborne viruses.

作者信息

Warren J C, Akers T G, Dubovi E J

出版信息

Appl Microbiol. 1969 Nov;18(5):893-6. doi: 10.1128/am.18.5.893-896.1969.

Abstract

Studies were undertaken to determine if a prewetting device (humidifier bulb) used in combination with an all glass impinger (AGI-30) would increase the recovery of airborne mengovirus-37A, vesicular stomatitis virus (VSV), and the S-13 coliphage. Suspensions of T3 coliphage with mengovirus-37A, VSV, or S-13 were aerosolized and collected by using the AGI-30-humidifier bulb combination to sample the aerosols before and after shifts in relative humidities (RH). These studies revealed the following. (i) At low RH values there was a 3 to 4 log increase in recovery of airborne T3 phage; (ii) concomitantly, the recovery of mengovirus-37A and VSV decreased; and (iii) only at the mid-range RH values was the recovery of S-13 enhanced. The prehumidification technique significantly increased the recovery of airborne T3 phage but decreased the recovery of the two animal viruses tested.

摘要

开展了多项研究,以确定与全玻璃冲击式采样器(AGI-30)联合使用的预湿装置(加湿器球)是否会提高空气传播的Mengovirus-37A、水泡性口炎病毒(VSV)和S-13大肠杆菌噬菌体的回收率。将T3大肠杆菌噬菌体与Mengovirus-37A、VSV或S-13的悬浮液雾化,并使用AGI-30-加湿器球组合在相对湿度(RH)变化前后对气溶胶进行采样以收集。这些研究结果如下:(i)在低RH值时,空气传播的T3噬菌体回收率增加3至4个对数;(ii)与此同时,Mengovirus-37A和VSV的回收率下降;(iii)仅在中等RH值时,S-13的回收率才会提高。预加湿技术显著提高了空气传播的T3噬菌体的回收率,但降低了所测试的两种动物病毒的回收率。

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