噬菌体T3在气溶胶中的失活：预加湿对从盐、蛋白胨和唾液溶液喷雾后存活的影响。
Inactivation of bacteriophage T3 in aerosols: effect of prehumidification on survival after spraying from solutions of salt, peptone, and saliva.
作者信息
Trouwborst T, Kuyper S
出版信息
Appl Microbiol. 1974 May;27(5):834-7. doi: 10.1128/am.27.5.834-837.1974.
Abstract
Coliphage T(3) was inactivated by a factor of 10(3) to 10(4) within 30 min after spraying from solutions of NaCl. Addition of peptone to the spray medium protected against inactivation at high relative humidity (RH), presumably by preventing surface inactivation. Prehumidification of the sample before collection had no effect on recovery if sprayed from solutions of NaCl, with or without peptone. If only peptone was present in the spray medium, prehumidification of the aerosol sample increased the recovery by a factor of 1,000 at low RH and by a factor of 5 at high RH. In aerosols sprayed from saliva, inactivation was nearly equal to that in peptone, with an increase of recovery after prehumidification by a factor of 1,000.
摘要
从氯化钠溶液中喷雾后30分钟内，大肠杆菌噬菌体T(3)被灭活10³至10⁴倍。向喷雾介质中添加蛋白胨可防止在高相对湿度（RH）下的灭活，这可能是通过防止表面灭活实现的。如果从含或不含蛋白胨的氯化钠溶液中喷雾，在采集前对样品进行预湿处理对回收率没有影响。如果喷雾介质中仅存在蛋白胨，在低相对湿度下，对气溶胶样品进行预湿处理可使回收率提高1000倍，在高相对湿度下提高5倍。从唾液中喷雾的气溶胶中，灭活情况几乎与蛋白胨中的相同，预湿处理后回收率提高1000倍。
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