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对原代小鼠肾细胞培养物中导致SV40诱导的染色体复制和有丝分裂的事件的分析。

Analysis of the events leading to SV40-induced chromosome replication and mitosis in primary mouse kidney cell cultures.

作者信息

May E, May P, Weil R

出版信息

Proc Natl Acad Sci U S A. 1971 Jun;68(6):1208-11. doi: 10.1073/pnas.68.6.1208.

Abstract

Abortive infection with simian virus 40 in confluent, "contact-inhibited", mouse kidney cell cultures was studied. The sequential events, in individual cells, are tentatively represented by the simplified scheme: (a) transcription of early virus-specific (messenger) RNA; (b) appearance of T-antigen; (c) [psychrosensitive event(s)]; (d) chromosome replication; (e) normal or abnormal mitosis. No evidence for the replication of viral progeny DNA was obtained. The sequence of the events (a)-(d) is analogous to that observed in contact-inhibited mouse kidney tissue culture cells during lytic infection with polyoma virus.

摘要

研究了在汇合的、“接触抑制”的小鼠肾细胞培养物中猿猴病毒40的流产感染。单个细胞中的一系列事件暂用简化模式表示如下:(a)早期病毒特异性(信使)RNA转录;(b)T抗原出现;(c)[低温敏感事件];(d)染色体复制;(e)正常或异常有丝分裂。未获得病毒子代DNA复制的证据。事件(a)-(d)的顺序与在用多瘤病毒进行裂解感染期间在接触抑制的小鼠肾组织培养细胞中观察到的顺序相似。

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