劳氏肉瘤病毒RNA与细胞DNA和病毒DNA的杂交
Hybridization of RNA from Rous sarcoma virus with cellular and viral DNA's.
作者信息
Yoshikawa-Fukada M, Ebert J D
出版信息
Proc Natl Acad Sci U S A. 1969 Nov;64(3):870-7. doi: 10.1073/pnas.64.3.870.
Abstract
The RNA of Rous sarcoma virus (RSV) hybridizes with DNA's isolated from all eukaryotes tested (animal and plant) with the possible exception of yeast. Bacterial DNA's show no homology to RSV-RNA. DNA's from the oncogenic viruses, adenovirus type 12 and SV40 show significant homology with RSV-RNA, but those from nononcogenic adenoviruses types 2 and 4 are not homologous.RSV-RNA hybridized to all of the DNA's examined has the same, or a closely similar, base composition. Moreover, the melting temperatures of all of the hybrids analyzed are very similar, further suggesting that a common base sequence in DNA functions in hybridization with RSV-RNA.
摘要
劳斯肉瘤病毒（RSV）的RNA能与从所有经测试的真核生物（动物和植物）中分离出的DNA杂交，酵母可能除外。细菌DNA与RSV-RNA无同源性。致癌病毒12型腺病毒和SV40的DNA与RSV-RNA显示出显著的同源性，但2型和4型非致癌腺病毒的DNA则无同源性。与所有检测的DNA杂交的RSV-RNA具有相同或极为相似的碱基组成。此外，所有分析的杂交体的解链温度都非常相似，这进一步表明DNA中的一个共同碱基序列在与RSV-RNA杂交中起作用。
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