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禽成髓细胞瘤病毒DNA与白血病鸡细胞内源性前病毒串联整合的证据。

Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells.

作者信息

Shoyab M, Dastoor M N, Baluda M A

出版信息

Proc Natl Acad Sci U S A. 1976 May;73(5):1749-53. doi: 10.1073/pnas.73.5.1749.

DOI:10.1073/pnas.73.5.1749
PMID:179099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430378/
Abstract

The integration site of avian myeloblastosis virus (AMV) proviral DNA in DNA from leukemia chicken myeloblasts has been studied by three sequential nucleic acid hybridizations that can localize the proviral DNA according to the repetitiveness of the adjacent cellular DNA regions. First, large denatured cellular DNA fragments (2.1 x 10(6) daltons) were reassociated and fractionated according to sequence reiteration frequenct. Next, DNA remaining single-stranded in each fraction was immobilized on nitrocellulose filters hybridized with an excess of unlabeled 70S RNA from Rous-associated virus-0 to saturate the endogenous proviral DNA sequences.

摘要

通过三次连续的核酸杂交研究了禽成髓细胞瘤病毒（AMV）前病毒DNA在白血病鸡成髓细胞DNA中的整合位点，这三次杂交可根据相邻细胞DNA区域的重复情况来定位前病毒DNA。首先，将大的变性细胞DNA片段（2.1×10⁶道尔顿）复性，并根据序列重复频率进行分级分离。接下来，将每个级分中仍为单链的DNA固定在硝酸纤维素滤膜上，与过量的来自劳氏相关病毒0的未标记70S RNA杂交，以使内源性前病毒DNA序列饱和。

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Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells.禽成髓细胞瘤病毒DNA与白血病鸡细胞内源性前病毒串联整合的证据。
Proc Natl Acad Sci U S A. 1976 May;73(5):1749-53. doi: 10.1073/pnas.73.5.1749.
2
Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells.禽成髓细胞瘤病毒DNA在白血病细胞中的整合与正常鸡细胞中内源性病毒DNA整合之间的差异。
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Studies on characterization of the integration sites of avian RNA tumor virus-specific DNA.
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Quantitative and qualitative differences in DNA complementary to avian myeloblastosis virus between normal and leukemic chicken cells.正常鸡细胞与白血病鸡细胞中与禽成髓细胞瘤病毒互补的DNA的定量和定性差异。
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引用本文的文献

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Measurement of proviral genes in uninfected and avian myeloblastosis virus-infected cells by hybridization with 3H-labeled complementary DNA probe excess.通过与过量的3H标记互补DNA探针杂交来测量未感染细胞和禽成髓细胞瘤病毒感染细胞中的前病毒基因。
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本文引用的文献

1
Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus.禽成髓细胞瘤病毒感染鸡细胞后新DNA序列的获得。
J Virol. 1974 Feb;13(2):331-9. doi: 10.1128/JVI.13.2.331-339.1974.
2
In vivo infectivity studies with avian mveloblastosis virus.禽成髓细胞瘤病毒的体内感染性研究
Virology. 1961 May;14:33-45. doi: 10.1016/0042-6822(61)90129-5.
3
An avian leukosis virus related to RSV(O): properties and evidence for helper activity.一种与劳斯肉瘤病毒（O型）相关的禽白血病病毒：特性及辅助活性证据
Virology. 1971 Jan;43(1):223-34. doi: 10.1016/0042-6822(71)90240-6.
4
The nucleotide sequence complexity of avian tumor virus RNA.禽肿瘤病毒RNA的核苷酸序列复杂性。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560-4. doi: 10.1073/pnas.71.9.3560.
5
Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells.禽成髓细胞瘤病毒DNA在白血病细胞中的整合与正常鸡细胞中内源性病毒DNA整合之间的差异。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3152-6. doi: 10.1073/pnas.71.8.3152.
6
Reliability of the RNA-DNA filter hybridization for the detection of oncornavirus-specific DNA sequences.用于检测肿瘤病毒特异性DNA序列的RNA-DNA滤膜杂交法的可靠性。
J Virol. 1974 Aug;14(2):225-30. doi: 10.1128/JVI.14.2.225-230.1974.
7
Presence in leukemic cells of avian myeloblastosis virus-specific DNA sequences absent in normal chicken cells.禽成髓细胞瘤病毒特异性DNA序列存在于白血病细胞中，而正常鸡细胞中不存在。
J Virol. 1974 Jul;14(1):47-9. doi: 10.1128/JVI.14.1.47-49.1974.
8
Synthesis of avian oncornavirus DNA in infected chicken cells.感染鸡细胞中禽肿瘤病毒DNA的合成。
J Virol. 1974 May;13(5):1005-13. doi: 10.1128/JVI.13.5.1005-1013.1974.
9
Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus.正常鸡细胞及禽成髓细胞瘤病毒转化细胞中肿瘤病毒DNA的整合状态
J Virol. 1973 Oct;12(4):721-32. doi: 10.1128/JVI.12.4.721-732.1973.
10
Early synthesis of virus-specific RNA and DNA in cells rapidly transformed with Rous sarcoma virus.用劳氏肉瘤病毒快速转化的细胞中病毒特异性RNA和DNA的早期合成。
Virology. 1973 Dec;56(2):532-48. doi: 10.1016/0042-6822(73)90056-1.