Spear P G, Kellejmroian B
J Virol. 1970 Feb;5(2):123-31. doi: 10.1128/JVI.5.2.123-131.1970.
Membranes prepared from HEp-2 cells infected with herpes simplex virus and free from soluble proteins, virus, ribosomes, and other cellular constituents were solubilized and subjected to electrophoresis on acrylamide gels. The electropherograms showed the following. (i) The synthesis of host proteins and glycoproteins ceases after infection. However, the spectrum of host proteins in membranes remains unaltered. (ii) Between 4 and 22 hr postinfection, at least four glycoproteins are synthesized and bound to the smooth cytoplasmic membranes. On electrophoresis, these glycoproteins form two major and two minor bands in the gel and migrate with proteins ranging from 50,000 to 100,000 daltons in molecular weight. (iii) The same glycoproteins are present in all membranes fractionated by density and in partially purified virus. The implications of the data are discussed.
用感染单纯疱疹病毒的HEP - 2细胞制备的、不含可溶性蛋白质、病毒、核糖体及其他细胞成分的膜，经溶解后在丙烯酰胺凝胶上进行电泳。电泳图谱显示如下：(i)感染后宿主蛋白质和糖蛋白的合成停止。然而，膜中宿主蛋白质的谱带保持不变。(ii)在感染后4至22小时之间，至少合成了四种糖蛋白并与光滑的细胞质膜结合。在电泳时，这些糖蛋白在凝胶中形成两条主要带和两条次要带，并与分子量在50,000至100,000道尔顿之间的蛋白质一起迁移。(iii)在通过密度分级分离的所有膜以及部分纯化的病毒中都存在相同的糖蛋白。讨论了这些数据的意义。
