Heine J W, Roizman B
J Virol. 1973 May;11(5):810-3. doi: 10.1128/JVI.11.5.810-813.1973.
Artificial mixtures of plasma membrane vesicles produced by microcavitation from infected and uninfected cells band at the same density on isopycnic centrifugation in sucrose density gradient. However, after reaction with antiviral antibody, the density of the infected cell plasma membrane vesicles increases, and the infected and uninfected cell membranes are quantitatively separable on isopycnic centrifugation. Plasma membrane vesicles prepared from cells doubly labeled before and after infection with radioactive amino acids and reacted with antibody banded at a high density. Polyacrylamide gel electropherograms show that the vesicles reacted with antibody consist of both host- and virus-specific membrane proteins. Microcavitation does not disrupt viral envelopes since infectivity is not affected by this procedure. We conclude that viral and cellular proteins in the plasma membrane preparations are contiguous.
通过微空化从感染细胞和未感染细胞产生的质膜囊泡人工混合物，在蔗糖密度梯度等密度离心中以相同密度形成条带。然而，与抗病毒抗体反应后，感染细胞质膜囊泡的密度增加，并且在等密度离心中感染细胞和未感染细胞的膜在数量上是可分离的。用放射性氨基酸在感染前后进行双重标记并与抗体反应的细胞制备的质膜囊泡在高密度下形成条带。聚丙烯酰胺凝胶电泳图谱显示，与抗体反应的囊泡由宿主特异性和病毒特异性膜蛋白组成。微空化不会破坏病毒包膜，因为感染性不受此过程影响。我们得出结论，质膜制剂中的病毒蛋白和细胞蛋白是相邻的。
