鼠伤寒沙门氏菌中的UGA无义突变。
UGA nonsense mutations in Salmonella typhimurium.
作者信息
Roth J R
出版信息
J Bacteriol. 1970 May;102(2):467-75. doi: 10.1128/jb.102.2.467-475.1970.
Abstract
Salmonella typhimurium strain LT-2 carries a weak UGA suppressor activity. This activity prevents the detection of some UGA mutants as auxotrophs and probably accounts for the rarity of his UGA mutants in this strain. A selection method is described which permits the isolation of these rare his UGA mutants. Map distribution of his UGA mutations is normal, and their polarity effects are indistinguishable from the polarity effects of amber and ochre mutations at similar locations. Isolation and properties of a prototrophic his UGA mutant are described. UGA mutants are common among lac mutants isolated from Salmonella strains carrying an F'lac episome. Apparently the suppressor activity is insufficient to prevent detection of lac UGA mutants. It is not yet clear whether the suppressor activity plays an important role in normal cell physiology.
摘要
鼠伤寒沙门氏菌LT-2菌株具有微弱的UGA抑制活性。这种活性会阻止一些UGA突变体被检测为营养缺陷型，这可能就是该菌株中his UGA突变体稀少的原因。本文描述了一种选择方法，该方法可用于分离这些罕见的his UGA突变体。his UGA突变的图谱分布正常，其极性效应与相似位置的琥珀突变和赭石突变的极性效应无法区分。本文还描述了一个原养型his UGA突变体的分离及其特性。UGA突变体在从携带F'lac附加体的沙门氏菌菌株中分离出的lac突变体中很常见。显然，这种抑制活性不足以阻止lac UGA突变体被检测到。目前尚不清楚这种抑制活性在正常细胞生理学中是否起重要作用。
相似文献
1
UGA nonsense mutations in Salmonella typhimurium.鼠伤寒沙门氏菌中的UGA无义突变。
J Bacteriol. 1970 May;102(2):467-75. doi: 10.1128/jb.102.2.467-475.1970.
2
Histidine regulation in Salmonella typhimurium. 8. Mutations of the hisT gene.鼠伤寒沙门氏菌中的组氨酸调控。8. hisT基因的突变
J Bacteriol. 1971 Oct;108(1):410-4. doi: 10.1128/jb.108.1.410-414.1971.
3
Suppression of amber and ochre mutants in Salmonella typhimurium by a mutant F'-1-gal factor carrying an ochre suppressor gene.携带赭石型抑制基因的F'-1-半乳糖突变因子对鼠伤寒沙门氏菌中琥珀型和赭石型突变体的抑制作用。
J Bacteriol. 1969 Oct;100(1):240-6. doi: 10.1128/jb.100.1.240-246.1969.
4
Translation and polarity in the histidine operon. 3. The isolation of prototrophic polar mutations.组氨酸操纵子中的翻译与极性。3. 原养型极性突变的分离。
J Mol Biol. 1967 Nov 28;30(1):109-24. doi: 10.1016/0022-2836(67)90247-1.
5
Procedure for identifying nonsense mutations.无义突变的鉴定程序。
J Bacteriol. 1968 Jul;96(1):215-20. doi: 10.1128/jb.96.1.215-220.1968.
6
Hemin-deficient mutants of Salmonella typhimurium.鼠伤寒沙门氏菌的血红素缺陷型突变体。
J Bacteriol. 1970 May;102(2):531-6. doi: 10.1128/jb.102.2.531-536.1970.
7
Histidinol dehydrogenase (his D) mutants of Salmonella typhimurium.鼠伤寒沙门氏菌的组氨醇脱氢酶（his D）突变体。
J Bacteriol. 1971 May;106(2):421-31. doi: 10.1128/jb.106.2.421-431.1971.
8
Low-level reading of the UGA triplet in Salmonella typhimurium.鼠伤寒沙门氏菌中UGA三联体的低水平阅读。
J Bacteriol. 1971 May;106(2):691-3. doi: 10.1128/jb.106.2.691-693.1971.
9
Histidine regulatory mutants in Salmonella typhimurium. IV. A positive selection for polar histidine-requiring mutants from histidine operator constitutive mutants.
J Mol Biol. 1967 Nov 28;30(1):81-95. doi: 10.1016/0022-2836(67)90245-8.
10
Mutations creating a new initiation point for expression of the histidine operon in Salmonella typhimurium.在鼠伤寒沙门氏菌中产生组氨酸操纵子表达新起始点的突变。
J Mol Biol. 1968 Jul 14;35(1):71-82. doi: 10.1016/s0022-2836(68)80037-3.
引用本文的文献
1
Differential Selection for Translation Efficiency Shapes Translation Machineries in Bacterial Species.细菌物种中翻译效率的差异选择塑造了翻译机制。
Microorganisms. 2024 Apr 10;12(4):768. doi: 10.3390/microorganisms12040768.
2
Stop Codon Usage as a Window into Genome Evolution: Mutation, Selection, Biased Gene Conversion and the TAG Paradox.终止密码子的使用作为窥探基因组进化的窗口：突变、选择、有偏基因转换和 TAG 悖论。
Genome Biol Evol. 2022 Aug 3;14(8). doi: 10.1093/gbe/evac115.
3
Unusual mammalian usage of TGA stop codons reveals that sequence conservation need not imply purifying selection.非典型哺乳动物对 TGA 终止密码子的使用表明，序列保守性不一定意味着纯化选择。
PLoS Biol. 2022 May 12;20(5):e3001588. doi: 10.1371/journal.pbio.3001588. eCollection 2022 May.
4
"Pseudo-pseudogenes" in bacterial genomes: Proteogenomics reveals a wide but low protein expression of pseudogenes in Salmonella enterica.细菌基因组中的“假假基因”：蛋白质基因组学揭示了沙门氏菌中假基因广泛但低水平的蛋白质表达。
Nucleic Acids Res. 2022 May 20;50(9):5158-5170. doi: 10.1093/nar/gkac302.
5
Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines.辉瑞/生物科技公司和莫德纳信使核糖核酸疫苗的详细剖析与批判性评估
Vaccines (Basel). 2021 Jul 3;9(7):734. doi: 10.3390/vaccines9070734.
6
Analysis of Stop Codons within Prokaryotic Protein-Coding Genes Suggests Frequent Readthrough Events.原核生物蛋白编码基因中终止密码子的分析表明频繁的通读事件。
Int J Mol Sci. 2021 Feb 14;22(4):1876. doi: 10.3390/ijms22041876.
7
Effective Population Size Predicts Local Rates but Not Local Mitigation of Read-through Errors.有效种群大小预测局部速率，但不能预测局部读通错误的缓解。
Mol Biol Evol. 2021 Jan 4;38(1):244-262. doi: 10.1093/molbev/msaa210.
8
In eubacteria, unlike eukaryotes, there is no evidence for selection favouring fail-safe 3' additional stop codons.在真细菌中，与真核生物不同，没有证据表明存在有利于无差错的 3' 附加终止密码子的选择。
PLoS Genet. 2019 Sep 17;15(9):e1008386. doi: 10.1371/journal.pgen.1008386. eCollection 2019 Sep.
9
Coevolution between Stop Codon Usage and Release Factors in Bacterial Species.细菌物种中终止密码子使用与释放因子之间的协同进化
Mol Biol Evol. 2016 Sep;33(9):2357-67. doi: 10.1093/molbev/msw107. Epub 2016 Jun 13.
10
Bacterial phylogenetic tree construction based on genomic translation stop signals.基于基因组翻译终止信号构建细菌系统发育树。
Microb Inform Exp. 2012 May 31;2(1):6. doi: 10.1186/2042-5783-2-6.
本文引用的文献
1
THE LINKAGE MAP OF SALMONELLA TYPHIMURIUM.鼠伤寒沙门氏菌的连锁图谱。
Genetics. 1965 Jun;51(6):897-913. doi: 10.1093/genetics/51.6.897.
2
GENES AND PROTEINS INVOLVED IN HISTIDINE BIOSYNTHESIS IN SALMONELLA.参与沙门氏菌组氨酸生物合成的基因和蛋白质
Brookhaven Symp Biol. 1964 Dec;17:15-52.
3
Chromosomal alterations affecting the regulation of histidine biosynthetic enzymes in Salmonella.影响沙门氏菌中组氨酸生物合成酶调控的染色体改变。
J Mol Biol. 1963 Jul;7:23-42. doi: 10.1016/s0022-2836(63)80016-9.
4
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
5
UGA: a third nonsense triplet in the genetic code.UGA：遗传密码中的第三个无义三联体。
Nature. 1967 Feb 4;213(5075):449-50. doi: 10.1038/213449a0.
6
A phage P22 gene controlling integration of prophage.一个控制原噬菌体整合的噬菌体P22基因。
Virology. 1967 Feb;31(2):207-16. doi: 10.1016/0042-6822(67)90164-x.
7
A proposal for a uniform nomenclature in bacterial genetics.细菌遗传学统一命名法的提议。
Genetics. 1966 Jul;54(1):61-76. doi: 10.1093/genetics/54.1.61.
8
A strong suppressor specific for UGA.一种对UGA具有特异性的强抑制子。
Nature. 1967 Apr 29;214(5087):452-3. doi: 10.1038/214452a0.
9
Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase.鼠伤寒沙门氏菌中的组氨酸调节突变体II。具有改变的组氨酰-tRNA合成酶的组氨酸调节突变体。
J Mol Biol. 1966 Dec 28;22(2):325-33. doi: 10.1016/0022-2836(66)90135-5.
10
Classification of aminotransferase (C gene) mutants in the histidine operon.组氨酸操纵子中氨基转移酶（C基因）突变体的分类
J Mol Biol. 1966 Nov 14;21(2):335-55. doi: 10.1016/0022-2836(66)90103-3.
Zotero 插件