Wever G H, Kit S, Dubbs D R
J Virol. 1970 May;5(5):578-85. doi: 10.1128/JVI.5.5.578-585.1970.
Simian virus 40 (SV40) can be rescued from certain SV40-transformed hamster cells by fusion with susceptible African green monkey kidney (CV-1) cells, in the presence of ultraviolet-irradiated Sendai virus. We have determined the sites in which SV40 is produced during rescue in these heterokaryons. To determine the sequence, nuclei were isolated from fused cells at various times after fusion, separated on sucrose-density gradients, and assayed for infectious center formation and virus content on CV-1 monolayers. Virus was first detected in the transformed nucleus (40 hr postfusion), and later associated with both transformed and susceptible nuclei (68 to 72 hr). Viral rescue apparently does not depend upon the transfer of SV40 deoxyribonucleic acid to a susceptible CV-1 nucleus, since the transformed nucleus is the primary site of virus production. The time course of certain cytological events in the rescue process and in productive infection was found to be similar.
在紫外线照射的仙台病毒存在的情况下，通过与易感的非洲绿猴肾（CV - 1）细胞融合，可从某些经猿猴病毒40（SV40）转化的仓鼠细胞中拯救出SV40。我们已经确定了在这些异核体拯救过程中SV40产生的位点。为了确定顺序，在融合后的不同时间从融合细胞中分离出细胞核，在蔗糖密度梯度上进行分离，并在CV - 1单层细胞上检测感染中心的形成和病毒含量。病毒首先在转化细胞核中被检测到（融合后40小时），随后与转化细胞核和易感细胞核都相关联（68至72小时）。病毒拯救显然不依赖于SV40脱氧核糖核酸转移到易感的CV - 1细胞核，因为转化细胞核是病毒产生的主要位点。发现拯救过程和生产性感染中某些细胞学事件的时间进程相似。
