Green M, Rokutanda M, Fujinaga K, Ray R K, Rokutanda H, Gurgo C
Proc Natl Acad Sci U S A. 1970 Sep;67(1):385-93. doi: 10.1073/pnas.67.1.385.
A highly active and stable DNA polymerase was found in purified preparations of two murine sarcoma viruses. Enzyme activity is not detected in most virus preparations unless they are treated with low concentrations of a nonionic detergent such as Nonidet P-40. The incorporation of labeled thymidine triphosphate requires all four deoxyribonucleoside triphosphates and either Mg(2+) or Mn(2+). Enzyme activity is proportional to virus concentration and is linear with time up to 90 min. That the template is RNA is suggested by the reduction in polymerase activity upon treatment of murine sarcoma virus with RNase, and by the absence of detectable amounts of DNA in the virus. That the product is DNA is shown by the incorporation of all four deoxyribo-nucleoside triphosphates into an acid-insoluble product which is stable in alkali, is destroyed by DNase, sediments in alkaline sucrose gradients with a sedimentation coefficient of 7 S, and bands in isopycnic CsCl gradients with a mean buoyant density of 1.700.
在两种鼠肉瘤病毒的纯化制剂中发现了一种高活性且稳定的DNA聚合酶。除非用低浓度的非离子去污剂（如诺乃洗涤剂P - 40）处理，否则在大多数病毒制剂中检测不到酶活性。标记的三磷酸胸腺嘧啶核苷的掺入需要所有四种脱氧核糖核苷三磷酸以及镁离子（Mg²⁺）或锰离子（Mn²⁺）。酶活性与病毒浓度成正比，并且在长达90分钟的时间内与时间呈线性关系。用核糖核酸酶（RNase）处理鼠肉瘤病毒后聚合酶活性降低，以及病毒中未检测到可检测量的DNA，这表明模板是RNA。通过将所有四种脱氧核糖核苷三磷酸掺入到一种酸不溶性产物中表明产物是DNA，该产物在碱中稳定，被脱氧核糖核酸酶（DNase）破坏，在碱性蔗糖梯度中沉降系数为7S，在等密度氯化铯梯度中条带的平均浮力密度为1.700。
