与劳氏肉瘤病毒和禽成髓细胞瘤病毒相关的脱氧核糖核酸聚合酶：酶及其产物的特性
Deoxyribonucleic acid polymerase associated with Rous sarcoma virus and avian myeloblastosis virus: properties of the enzyme and its product.
作者信息
Garapin A C, McDonnell J P, Levinson W, Quintrell N, Fanshier L, Bishop J M
出版信息
J Virol. 1970 Nov;6(5):589-98. doi: 10.1128/JVI.6.5.589-598.1970.
Abstract
Deoxyribonucleic acid (DNA) polymerase activity can be elicited in purified preparations of avian myeloblastosis virus and Rous sarcoma virus (Schmidt-Ruppin strain) by treatment with nonionic detergent. The enzyme(s) and its synthetic products appear to be virion-associated. Enzymatic activity can be inhibited by pretreatment with either ribonuclease (8- to 10-fold inhibition) or actinomycin D (twofold inhibition). By contrast, rifampin has little, if any effect. The enzyme(s) synthesizes two primary products, a ribonucleic acid (RNA):DNA hybrid and DNA which is free of RNA. The results of both zonal and equilibrium centrifugation indicate that nascent chains of DNA are associated with the 70S viral RNA. It is concluded that at least two enzymatic activities are under study: transcription of DNA from viral RNA, and subsequent, additional synthesis of DNA, utilizing product of the initial reaction as template.
摘要
通过用非离子去污剂处理，可在禽成髓细胞瘤病毒和劳氏肉瘤病毒（施密特 - 鲁平株）的纯化制剂中引发脱氧核糖核酸（DNA）聚合酶活性。该酶及其合成产物似乎与病毒粒子相关。用核糖核酸酶（8至10倍抑制）或放线菌素D（两倍抑制）预处理可抑制酶活性。相比之下，利福平几乎没有作用。该酶合成两种主要产物，一种核糖核酸（RNA）：DNA杂交体和不含RNA的DNA。区带离心和平衡离心的结果表明，新生的DNA链与70S病毒RNA相关。得出的结论是，至少正在研究两种酶活性：从病毒RNA转录DNA，以及随后利用初始反应产物作为模板额外合成DNA。
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