含RNA肿瘤病毒的DNA合成
DNA synthesis by RNA-containing tumor viruses.
作者信息
Scolnick E M, Aaronson S A, Todaro G J
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):1034-41. doi: 10.1073/pnas.67.2.1034.
Abstract
Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties.
摘要
小鼠白血病病毒（劳舍尔株和莫洛尼株）、肉瘤病毒（柯斯顿株）以及小鼠乳腺肿瘤病毒都含有一种依赖RNA的DNA聚合酶。三磷酸脱氧核糖核苷的最佳掺入发生在临界去污剂（曲拉通X-100）浓度（0.010 - 0.014%）时。当去污剂浓度高于最佳浓度时，病毒粒子会被破坏且酶活性丧失。病毒粒子、酶活性以及新合成的DNA在蔗糖梯度中都一起沉降。到目前为止，仅在具有致癌特性的RNA病毒中发现了这种酶活性。
相似文献
1
DNA synthesis by RNA-containing tumor viruses.含RNA肿瘤病毒的DNA合成
Proc Natl Acad Sci U S A. 1970 Oct;67(2):1034-41. doi: 10.1073/pnas.67.2.1034.
2
Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses.致癌RNA病毒中DNA指导的DNA聚合酶产物的特性分析
Nature. 1970 Aug 8;227(5258):563-7. doi: 10.1038/227563a0.
3
Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses.RNA肿瘤病毒的致癌机制。I. 鼠肉瘤病毒中的一种RNA依赖性DNA聚合酶。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):385-93. doi: 10.1073/pnas.67.1.385.
4
DNA-directed DNA polymerase activity in oncogenic RNA viruses.致癌RNA病毒中的DNA指导的DNA聚合酶活性。
Nature. 1970 Sep 5;227(5262):1029-31. doi: 10.1038/2271029a0.
5
RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.五种RNA病毒中的RNA依赖性DNA聚合酶活性：二价阳离子需求
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96. doi: 10.1073/pnas.67.4.1789.
6
Defective mouse sarcoma virus deficient in DNA polymerase activity.缺乏DNA聚合酶活性的缺陷型小鼠肉瘤病毒。
J Gen Virol. 1972 Aug;16(2):223-6. doi: 10.1099/0022-1317-16-2-223.
7
Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses.合成的DNA-RNA杂交体和RNA-RNA双链体作为致癌RNA病毒聚合酶的模板。
Nature. 1970 Oct 31;228(5270):430-2. doi: 10.1038/228430a0.
8
Endonucleolytic DNase in oncorna viruses: role of mycoplasma.致肿瘤RNA病毒中的核酸内切脱氧核糖核酸酶：支原体的作用
Virology. 1974 Jan;57(1):217-26. doi: 10.1016/0042-6822(74)90122-6.
9
Separation of ribonuclease H and RNA directed DNA polymerase (reverse transcriptase) of murine type-C RNA tumor viruses.鼠C型RNA肿瘤病毒的核糖核酸酶H与RNA指导的DNA聚合酶（逆转录酶）的分离
Proc Natl Acad Sci U S A. 1974 May;71(5):1871-6. doi: 10.1073/pnas.71.5.1871.
10
DNA polymerase activity associated with RNA tumor viruses.与RNA肿瘤病毒相关的DNA聚合酶活性
Proc Natl Acad Sci U S A. 1970 Sep;67(1):143-7. doi: 10.1073/pnas.67.1.143.
引用本文的文献
1
Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems.细菌表达系统中逆转录酶和DNA聚合酶的生产。
Bioengineering (Basel). 2024 Jul 18;11(7):727. doi: 10.3390/bioengineering11070727.
2
Prime editing: Mechanism insight and recent applications in plants.碱基编辑：机制洞察与植物中的最新应用。
Plant Biotechnol J. 2024 Jan;22(1):19-36. doi: 10.1111/pbi.14188. Epub 2023 Oct 4.
3
Bst polymerase - a humble relative of Taq polymerase.Bst聚合酶——Taq聚合酶的一个低调的同类。
Comput Struct Biotechnol J. 2023 Sep 12;21:4519-4535. doi: 10.1016/j.csbj.2023.09.008. eCollection 2023.
4
How Well Does Evolution Explain Endogenous Retroviruses?-A Lakatosian Assessment.进化在多大程度上能解释内源性逆转录病毒？——基于拉卡托斯评价模式的评估。
Viruses. 2021 Dec 22;14(1):14. doi: 10.3390/v14010014.
5
M-MuLV reverse transcriptase: Selected properties and improved mutants.莫洛尼鼠白血病病毒逆转录酶：选定特性及改良突变体
Comput Struct Biotechnol J. 2021 Nov 22;19:6315-6327. doi: 10.1016/j.csbj.2021.11.030. eCollection 2021.
6
References.参考文献。
Perspect Med Virol. 1986;2:209-245. doi: 10.1016/S0168-7069(08)70043-0. Epub 2008 May 29.
7
Deoxyribonucleic acid polymerase activity associated with strain MC29 tumor virus.与MC29菌株肿瘤病毒相关的脱氧核糖核酸聚合酶活性。
J Virol. 1971 Feb;7(2):214-20. doi: 10.1128/JVI.7.2.214-220.1971.
8
Deoxyribonucleic acid polymerase activity associated with a plasma particulate fraction from patients with chronic lymphocytic leukemia.与慢性淋巴细胞白血病患者血浆微粒部分相关的脱氧核糖核酸聚合酶活性
J Virol. 1971 Feb;7(2):221-6. doi: 10.1128/JVI.7.2.221-226.1971.
9
In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).与纯化的兔珠蛋白信使核糖核酸互补的DNA的体外合成（RNA依赖性DNA聚合酶-网织红细胞-血红蛋白密度梯度离心-寡聚（dT）引物）
Proc Natl Acad Sci U S A. 1972 Jan;69(1):264-8. doi: 10.1073/pnas.69.1.264.
10
Synthesis of DNA complements of natural RNAs: a general approach.天然RNA的DNA互补序列的合成：一种通用方法。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2843-5. doi: 10.1073/pnas.68.11.2843.
本文引用的文献
1
2
The detection and study of tumor viruses with the electron microscope.利用电子显微镜对肿瘤病毒进行检测与研究。
Cancer Res. 1960 Jun;20:712-27.
3
Bioactivity and virions in the blood of mice with mammary tumor virus.患有乳腺肿瘤病毒的小鼠血液中的生物活性和病毒粒子。
J Natl Cancer Inst. 1970 Apr;44(4):965-73.
4
5
Attenuation of the Rauscher murine leukemia virus through serial passages in tissue culture.通过在组织培养中连续传代使劳氏鼠白血病病毒减毒。
Natl Cancer Inst Monogr. 1966 Sep;22:685-700.
6
Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores.痘苗病毒中的核糖核酸合成。I. 痘苗病毒核心中RNA的合成与释放机制。
J Mol Biol. 1970 May 28;50(1):1-18. doi: 10.1016/0022-2836(70)90100-2.
7
Experimental transmission of feline fibrosarcoma to cats and dogs.猫纤维肉瘤向猫和狗的实验性传播。
Nature. 1970 May 30;226(5248):807-9. doi: 10.1038/226807a0.
8
Infection of human embryonic cell cultures with the Rauscher murine leukemia virus.劳氏鼠白血病病毒对人胚胎细胞培养物的感染。
Cancer Res. 1969 Oct;29(10):1886-8.
9
Isolation of an E. coli strain with a mutation affecting DNA polymerase.分离出一株具有影响DNA聚合酶突变的大肠杆菌菌株。
Nature. 1969 Dec 20;224(5225):1164-6. doi: 10.1038/2241164a0.
10
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.劳氏肉瘤病毒病毒粒子中的RNA依赖性DNA聚合酶。
Nature. 1970 Jun 27;226(5252):1211-3. doi: 10.1038/2261211a0.
Zotero 插件