无缺陷腺病毒2-猴病毒40杂交病毒的研究。IX. 猴病毒40早期区域的模板拓扑结构。
Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IX. Template topography in the early region of simian virus 40.
作者信息
Patch C T, Lewis A M, Levine A S
出版信息
J Virol. 1974 Mar;13(3):677-89. doi: 10.1128/JVI.13.3.677-689.1974.
Abstract
The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relative amounts of early and late SV40 template in the DNA of each virus were determined by RNA-DNA hybridization with late lytic SV40 RNA, which contains sequences complementary to both templates. From the distribution of early and late templates in the five overlapping SV40 segments, we conclude that either the entire early region of SV40 is symmetrically transcribed in vivo, or, more probably, that the early SV40 templates are not contiguous.
摘要
五种无缺陷腺病毒2（Ad2）-猴病毒40（SV40）杂交病毒的DNA含有野生型SV40 DNA早期区域的重叠片段。这五种病毒的互补DNA链已在等密度氯化铯梯度中用合成多核糖核苷酸分离。通过与晚期裂解性SV40 RNA进行RNA-DNA杂交，确定了每种病毒DNA中早期和晚期SV40模板的相对量，晚期裂解性SV40 RNA包含与两种模板互补的序列。根据早期和晚期模板在五个重叠的SV40片段中的分布，我们得出结论：要么SV40的整个早期区域在体内对称转录，要么更有可能的是，早期SV40模板不连续。
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本文引用的文献
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Asymmetric distribution of the transcribing regions on the complementary strands of coliphage lambda DNA.大肠杆菌噬菌体λDNA互补链上转录区域的不对称分布。
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10
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