Murad F, Manganiello V, Vaughan M
Proc Natl Acad Sci U S A. 1971 Apr;68(4):736-9. doi: 10.1073/pnas.68.4.736.
A protein-binding assay for guanosine 3':5'-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as little as 0.5-1 pmol of cyclic GMP. In some instances, it is possible to assay the nucleotide in urine or deproteinized extracts of tissue without purification. For other samples, a simple method for separating and purifying cyclic AMP and cyclic GMP is described.
本文描述了一种用于检测3':5'-环磷酸鸟苷的蛋白质结合分析方法,该方法使用了龙虾尾肌的酶制剂。四种不同酶制剂对环磷酸鸟苷的结合常数为2-10 nM。该方法可在数天内建立,能够检测低至0.5-1皮摩尔的环磷酸鸟苷。在某些情况下,无需纯化即可检测尿液或组织脱蛋白提取物中的核苷酸。对于其他样品,本文还描述了一种分离和纯化环磷酸腺苷和环磷酸鸟苷的简单方法。