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1
A simple, sensitive protein-binding assay for guanosine 3':5'-monophosphate.一种用于3':5'-环磷酸鸟苷的简单、灵敏的蛋白质结合测定法。
Proc Natl Acad Sci U S A. 1971 Apr;68(4):736-9. doi: 10.1073/pnas.68.4.736.
2
Radioimmunoassay for adenosine 3', 5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate in human blood, urine, and cerebrospinal fluid.人血液、尿液和脑脊液中3',5'-环磷酸腺苷和3',5'-环磷酸鸟苷的放射免疫测定法。
Clin Chem. 1977 Mar;23(3):576-80.
3
Simultaneous determination of guanine nucleotides in rat brain by high-performance liquid chromatography with dual-electrochemical detection.高效液相色谱-双电化学检测法同时测定大鼠脑中鸟嘌呤核苷酸
Anal Biochem. 1984 Nov 1;142(2):395-9. doi: 10.1016/0003-2697(84)90482-2.
4
A new enzymatic assay for guanosine 3':5'-cyclic monophosphate and its application to the ductus deferens of the rat.一种新的环磷酸鸟苷酶促测定法及其在大鼠输精管中的应用。
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1721-5. doi: 10.1073/pnas.70.6.1721.
5
Rapid radioimmunoassay for guanosine 3',5'-cyclic monophosphate using tritiated ligand.使用氚标记配体的3',5'-环磷酸鸟苷快速放射免疫测定法。
J Biochem. 1975 Jul;78(1):131-7.
6
Analysis of cyclic AMP and cyclic GMP by enzymic cycling procedures.通过酶循环法分析环磷酸腺苷和环磷酸鸟苷。
Adv Cyclic Nucleotide Res. 1972;2:63-80.
7
Simultaneous isolation of adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) in small tissue samples.在小组织样本中同时分离3',5'-环磷酸腺苷（cAMP）和3',5'-环磷酸鸟苷（cGMP）。
Anal Biochem. 1974 May;59(1):63-8. doi: 10.1016/0003-2697(74)90009-8.
8
An improved protein binding assay for guanosine-3',5'-monophosphate using a binding protein from the pupa of the silkmoth, Bombyx mori L.一种利用家蚕（Bombyx mori L.）蛹的结合蛋白改进的鸟苷-3',5'-单磷酸蛋白结合测定法。
Acta Biol Med Ger. 1976;35(5):543-52.
9
Kinetic parameters and renal clearances of plasma adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate in man.人体中血浆3',5'-单磷酸腺苷和3',5'-单磷酸鸟苷的动力学参数及肾清除率
J Clin Invest. 1970 Dec;49(12):2222-36. doi: 10.1172/JCI106441.
10
Adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate: a simultaneous protein binding assay.3',5'-环磷酸腺苷和3',5'-环磷酸鸟苷：一种同步蛋白质结合测定法
Biochim Biophys Acta. 1971 Nov 12;252(2):397-400. doi: 10.1016/0304-4165(71)90020-1.

引用本文的文献

1
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.人及大鼠胰岛中环核苷酸磷酸二酯酶的表达和调节。
PLoS One. 2010 Dec 1;5(12):e14191. doi: 10.1371/journal.pone.0014191.
2
A short history of cGMP, guanylyl cyclases, and cGMP-dependent protein kinases.环磷酸鸟苷（cGMP）、鸟苷酸环化酶及cGMP依赖性蛋白激酶简史
Handb Exp Pharmacol. 2009(191):1-14. doi: 10.1007/978-3-540-68964-5_1.
3
Nitric oxide leads to prized NObility: background to the work of Ferid Murad.一氧化氮带来珍贵的“诺贝尔”殊荣：费里德·穆拉德的研究背景
Tex Heart Inst J. 1999;26(1):1-5.
4
125I-8-L-arginine vasopressin binding to human mononuclear phagocytes.125I-8-L-精氨酸加压素与人单核吞噬细胞的结合
J Clin Invest. 1981 Aug;68(2):374-81. doi: 10.1172/jci110265.
5
Brain cyclic nucleotide and energy metabolite responses to subanesthetic and anesthetic concentrations of halothane.脑内环核苷酸和能量代谢产物对氟烷亚麻醉浓度和麻醉浓度的反应。
Experientia. 1980 Jun 15;36(6):655-6. doi: 10.1007/BF01970119.
6
Guanosine 3':5'-monophosphate-dependent protein kinases in mammalian tissues.哺乳动物组织中3':5'-环磷酸鸟苷依赖性蛋白激酶
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4037-41. doi: 10.1073/pnas.71.10.4037.
7
A rapid and simple procedure for the determination of guanosine 3',5'-monophosphate by use of the protein-binding method.一种采用蛋白质结合法快速简便地测定3',5'-环磷酸鸟苷的方法。
Naunyn Schmiedebergs Arch Pharmacol. 1974;284(1):55-61. doi: 10.1007/BF00499972.
8
Estrogen-related increases in uterine guanosine 3':5'-cyclic momophosphate levels.雌激素相关的子宫3':5'-环磷酸鸟苷水平升高。
Proc Natl Acad Sci U S A. 1974 May;71(5):1866-70. doi: 10.1073/pnas.71.5.1866.
9
Guanosine 3':5'-cyclic monophosphate and the action of insulin and acetylcholine.3':5'-环磷酸鸟苷以及胰岛素与乙酰胆碱的作用
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2443-7. doi: 10.1073/pnas.70.8.2443.
10
Alteration of vein cyclic 3':5' nucleotide concentrations during changes in contractility.收缩性改变期间静脉环3':5'核苷酸浓度的变化
Proc Natl Acad Sci U S A. 1974 Mar;71(3):815-9. doi: 10.1073/pnas.71.3.815.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE ASSAY OF ADENOSINE-3' ,5'-PHOSPHATE IN SKELETAL MUSCLE.骨骼肌中3' ,5'-磷酸腺苷的测定
Biochemistry. 1964 Aug;3:1040-4. doi: 10.1021/bi00896a005.
3
Isolation of adenosine 3', 5'-monophosphate and guanosine 3', 5'-monophosphate from rat urine.从大鼠尿液中分离3',5'-环磷酸腺苷和3',5'-环磷酸鸟苷。
Biochem Biophys Res Commun. 1963 May 22;11:330-4. doi: 10.1016/0006-291x(63)90566-7.
4
Factors affecting the activity of muscle glycogen synthetase. 3. The reaction with adenosine triphosphate Mg++, and cyclic 3'5'-adenosine monophosphate.影响肌肉糖原合成酶活性的因素。3. 与三磷酸腺苷镁离子及环磷酸腺苷的反应
Arch Biochem Biophys. 1966 Sep 26;116(1):39-43. doi: 10.1016/0003-9861(66)90009-9.
5
Effects of parathyroid hormone on plasma and urinary adenosine 3',5'-monophosphate in man.甲状旁腺激素对人体血浆和尿中3',5'-单磷酸腺苷的影响。
J Clin Invest. 1970 Dec;49(12):2387-95. doi: 10.1172/JCI106458.
6
Cyclic nucleotide-dependent protein kinases. VI. Isolation and partial purification of a protein kinase activated by guanosine 3',5'-monophosphate.环核苷酸依赖性蛋白激酶。VI. 由3',5'-环磷酸鸟苷激活的蛋白激酶的分离及部分纯化。
J Biol Chem. 1970 May 25;245(10):2493-8.
7
Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3',5'-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom.环核苷酸依赖性蛋白激酶。IV. 3',5'-环磷酸腺苷依赖性蛋白激酶在动物界各组织和门类中的广泛存在。
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1349-55. doi: 10.1073/pnas.64.4.1349.
8
Conditions for the formation, partial purification and assay of an inhibitor of adenosine 3',5'-monophosphate.3',5'-单磷酸腺苷抑制剂的形成、部分纯化及测定条件
Biochim Biophys Acta. 1969 Dec 30;192(3):430-45. doi: 10.1016/0304-4165(69)90392-4.
9
Effects of guanosine 3',5'-monophosphate on glycerol production and accumulation of adenosine 3',5'-monophosphate by fat cells.3',5'-环磷酸鸟苷对脂肪细胞甘油生成及3',5'-环磷酸腺苷积累的影响。
J Biol Chem. 1970 Jul 10;245(13):3352-60.
10
An assay for adenosine 3',5'-cyclic monophosphate based on the association of the nucleotide with a partially purified binding protein.一种基于核苷酸与部分纯化的结合蛋白结合的3',5'-环磷酸腺苷检测方法。
Biochemistry. 1970 Oct 13;9(21):4223-9. doi: 10.1021/bi00823a026.

一种用于3':5'-环磷酸鸟苷的简单、灵敏的蛋白质结合测定法。

A simple, sensitive protein-binding assay for guanosine 3':5'-monophosphate.

作者信息

Murad F, Manganiello V, Vaughan M

出版信息

Proc Natl Acad Sci U S A. 1971 Apr;68(4):736-9. doi: 10.1073/pnas.68.4.736.

DOI:10.1073/pnas.68.4.736
PMID:4323786
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389031/
Abstract

A protein-binding assay for guanosine 3':5'-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as little as 0.5-1 pmol of cyclic GMP. In some instances, it is possible to assay the nucleotide in urine or deproteinized extracts of tissue without purification. For other samples, a simple method for separating and purifying cyclic AMP and cyclic GMP is described.

摘要

本文描述了一种用于检测3':5'-环磷酸鸟苷的蛋白质结合分析方法，该方法使用了龙虾尾肌的酶制剂。四种不同酶制剂对环磷酸鸟苷的结合常数为2-10 nM。该方法可在数天内建立，能够检测低至0.5-1皮摩尔的环磷酸鸟苷。在某些情况下，无需纯化即可检测尿液或组织脱蛋白提取物中的核苷酸。对于其他样品，本文还描述了一种分离和纯化环磷酸腺苷和环磷酸鸟苷的简单方法。