副流感病毒感染的临床及免疫荧光诊断观察
Observations on clinical and immunofluorescent diagnosis of parainfluenza virus infections.
作者信息
Gardner P S, McQuillin J, McGuckin R, Ditchburn R K
出版信息
Br Med J. 1971 Apr 3;2(5752):7-12. doi: 10.1136/bmj.2.5752.7.
Immunofluorescent techniques have been applied to nasopharyngeal secretions for the rapid diagnosis of parainfluenza virus types 1, 2, and 3 infections. Seventy-five infections were found by isolation techniques; 55 of these had nasopharyngeal secretions taken and 53 were positive by direct examination. A comparison of the results of 60 neutralization tests with immunofluorescence applied to monkey kidney isolations showed complete agreement. Immunofluorescence appeared to be a satisfactory method for differentiating the various haemadsorption viruses. The importance of parainfluenza viruses and respiratory syncytial virus in croup was noted and the association of the parainfluenza viruses with acute respiratory virus infection was confirmed. The clinical relationship between respiratory syncytial virus and parainfluenza virus type 3 is discussed.
免疫荧光技术已应用于鼻咽分泌物，用于快速诊断1、2和3型副流感病毒感染。通过分离技术发现了75例感染；其中55例采集了鼻咽分泌物，53例经直接检查呈阳性。对60份中和试验结果与应用于猴肾分离株的免疫荧光结果进行比较，结果完全一致。免疫荧光似乎是区分各种血细胞吸附病毒的一种令人满意的方法。注意到副流感病毒和呼吸道合胞病毒在哮吼中的重要性，并证实了副流感病毒与急性呼吸道病毒感染的关联。还讨论了呼吸道合胞病毒与3型副流感病毒之间的临床关系。
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