Wong D T, Welliver R C, Riddlesberger K R, Sun M S, Ogra P L
J Clin Microbiol. 1982 Jul;16(1):164-7. doi: 10.1128/jcm.16.1.164-167.1982.
The indirect immunofluorescent-antibody (IFA) assay for detection of parainfluenza virus antigen was performed on samples of nasopharyngeal secretions obtained from infants and children with various respiratory illnesses to determine the usefulness of the IFA assay for rapid diagnosis of parainfluenza virus infection. Ninety-four samples of nasopharyngeal secretions were obtained from 78 children during a community outbreak of parainfluenza virus infection. Application of the IFA assay revealed the presence of parainfluenza antigen in 67 of the 94 samples. When these same specimens were inoculated into tissue culture, the presence of parainfluenza virus was confirmed in 62 (93%) of the 67 IFA-positive specimens by hemadsorption on tissue culture monolayers (31 cases), presence of parainfluenza hemagglutinin in infected tissue culture fluids (23 cases), or by other methods (8 cases). Only four IFA-negative specimens were subsequently shown to be positive for parainfluenza virus by tissue culture infectivity. The IFA assay provided a more rapid and more accurate method for diagnosis of parainfluenza infection in children than did routine tissue culture methods employed currently.
为确定间接免疫荧光抗体（IFA）检测法对副流感病毒感染快速诊断的实用性，对患有各种呼吸道疾病的婴幼儿的鼻咽分泌物样本进行了副流感病毒抗原检测。在社区副流感病毒感染暴发期间，从78名儿童身上采集了94份鼻咽分泌物样本。IFA检测法显示，94份样本中有67份存在副流感病毒抗原。当将这些相同的标本接种到组织培养中时，通过对组织培养单层进行血细胞吸附（31例）、检测感染的组织培养液中副流感血凝素的存在（23例）或其他方法（8例），在67份IFA阳性标本中的62份（93%）中确认了副流感病毒的存在。通过组织培养感染性检测，仅4份IFA阴性标本随后被证明副流感病毒呈阳性。与目前使用的常规组织培养方法相比，IFA检测法为儿童副流感感染的诊断提供了一种更快速、更准确的方法。
