Calvin M, Joss U R, Hackett A J, Owens R B
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1441-3. doi: 10.1073/pnas.68.7.1441.
It is shown that rifampicin, and especially the related antibiotic 2',6'-dimethyl-N(4')-benzyl-N(4')- [desmethyl]rifampicin (DMB-rifampicin) can inhibit focus formation by Moloney sarcoma virus on BALB/3T3 tissue cultures. At 10 mug/ml DMB-rifampicin totally inhibits focus formation while reducing virus replication by at least a factor of fifty and cell proliferation by only a factor of three. These observations, taken together with those of others, suggest a role for an RNA-dependent DNA polymerase and the gene for its synthesis both in normal cell processes and in the transformation process.
已表明利福平,尤其是相关抗生素2',6'-二甲基-N(4')-苄基-N(4')-[去甲基]利福平(DMB-利福平)可抑制莫洛尼肉瘤病毒在BALB/3T3组织培养物上形成病灶。在10微克/毫升时,DMB-利福平完全抑制病灶形成,同时使病毒复制减少至少五十倍,细胞增殖仅减少三倍。这些观察结果与其他观察结果一起,提示一种依赖RNA的DNA聚合酶及其合成基因在正常细胞过程和转化过程中均发挥作用。