双链病毒RNA对培养的哺乳动物细胞的作用。
Effect of double-stranded viral RNA on mammalian cells in culture.
作者信息
Cordell-Stewart B, Taylor M W
出版信息
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1326-30. doi: 10.1073/pnas.68.6.1326.
Abstract
During bovine enterovirus infection of Ehrlich ascites tumor cells, large amounts of double-stranded RNA accumulate. Addition of this double-stranded RNA to uninfected cells leads to rapid cell death. This is not a result of infectious virus production. Neither single-stranded RNA nor heat-denatured double-stranded RNA has this effect. Similar experiments with synthetic double-stranded polymers, poly(I).poly(C) and poly(A).poly(U), show that they are only slightly toxic at the concentrations used. The effect of the double-stranded RNA is nonspecific for cells of different origins. The implications of this finding in relation to the cytopathic effects of picornavirus and to cancer chemotherapy are discussed.
摘要
在牛肠道病毒感染艾氏腹水瘤细胞的过程中，会积累大量双链RNA。将这种双链RNA添加到未感染的细胞中会导致细胞迅速死亡。这不是传染性病毒产生的结果。单链RNA和热变性双链RNA都没有这种作用。用合成双链聚合物聚肌苷酸-聚胞苷酸（poly(I).poly(C)）和聚腺苷酸-聚尿苷酸（poly(A).poly(U)）进行的类似实验表明，在所用浓度下它们只有轻微毒性。双链RNA的作用对不同来源的细胞是非特异性的。本文讨论了这一发现与小核糖核酸病毒的细胞病变效应以及癌症化疗的关系。
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