Carter W A, Levy H B
Science. 1967 Mar 10;155(3767):1254-7. doi: 10.1126/science.155.3767.1254.
Rapidly labeled RNA of mouse L cells and labeled RNA of Mengo virus, unlike cellular RNA labeled under steady-state conditions, form detectable complexes with L-cell ribosomes. These ribosome-RNA complexes formed in vitro appear analogous to those assembled during polysome formation in vivo. When ribosomes are prepared from L cells exposed to homologous interferon, their capacity to associate with cell messenger is preserved, while their ability to interact with viral RNA is markedly reduced. The ribosomes from cells exposed to interferon are thus altered selectively to permit only certain messages to be bound and translated.
与在稳态条件下标记的细胞RNA不同，小鼠L细胞的快速标记RNA和门戈病毒的标记RNA能与L细胞核糖体形成可检测到的复合物。这些体外形成的核糖体-RNA复合物似乎类似于体内多核糖体形成过程中组装的复合物。当从暴露于同源干扰素的L细胞中制备核糖体时，它们与细胞信使结合的能力得以保留，而与病毒RNA相互作用的能力则显著降低。因此，暴露于干扰素的细胞中的核糖体发生了选择性改变，仅允许某些信使被结合和翻译。
