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1
Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli.大肠杆菌乳糖操纵子体外转录的起始与抑制机制
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828-32. doi: 10.1073/pnas.68.8.1828.
2
Lac DNA, RNA polymerase and cyclic AMP receptor protein, cyclic AMP, lac repressor and inducer are the essential elements for controlled lac transcription.乳糖操纵子DNA、RNA聚合酶、环腺苷酸受体蛋白、环腺苷酸、乳糖阻遏物和诱导物是乳糖转录调控的基本要素。
Nat New Biol. 1971 Jun 2;231(22):139-42. doi: 10.1038/newbio231139a0.
3
An adenosine 3':5'-cyclic monophosphate-binding protein that acts on the transcription process.一种作用于转录过程的3':5'-环磷酸腺苷结合蛋白。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):215-8. doi: 10.1073/pnas.68.1.215.
4
Effect of a low-molecular-weight DNA binding protein, H1 factor, on the in vitro transcription of the lactose operon in Escherichia coli.一种低分子量DNA结合蛋白H1因子对大肠杆菌乳糖操纵子体外转录的影响。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):333-7. doi: 10.1073/pnas.72.1.333.
5
[How does the lac repressor recognize the lac operator?].[乳糖阻遏蛋白是如何识别乳糖操纵基因的？]
Hoppe Seylers Z Physiol Chem. 1972 May;353(5):693-4.
6
Dual promoter control of the Escherichia coli lactose operon.大肠杆菌乳糖操纵子的双启动子控制
Cell. 1984 Nov;39(1):173-80. doi: 10.1016/0092-8674(84)90203-4.
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Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli.大肠杆菌色氨酸操纵子阻遏蛋白的检测与分离
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8
Regulation of lac mRNA synthesis in a soluble cell-free system.可溶性无细胞体系中乳糖操纵子mRNA合成的调控
Nat New Biol. 1971 Mar 10;230(10):37-40. doi: 10.1038/newbio230037a0.
9
Genetic mapping of a new promoter for the lac operon.乳糖操纵子新启动子的遗传图谱分析。
J Mol Biol. 1975 Apr 5;93(2):311-7. doi: 10.1016/0022-2836(75)90134-5.
10
Turning on the lactose genes.开启乳糖基因。
Nat New Biol. 1971 Mar 10;230(10):33. doi: 10.1038/newbio230033a0.

引用本文的文献

1
Stochastic simulation and statistical inference platform for visualization and estimation of transcriptional kinetics.用于转录动力学可视化和估计的随机模拟和统计推断平台。
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Streamlining the preparation of "endotoxin-free" ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase.利用自诱导培养基简化“无内毒素”ClearColi细胞提取物的制备，用于治疗性蛋白crisantaspase的无细胞蛋白质合成。
Synth Syst Biotechnol. 2019 Dec 13;4(4):220-224. doi: 10.1016/j.synbio.2019.11.003. eCollection 2019 Dec.
3
Deletion mapping a eukaryotic promoter.真核生物启动子的缺失作图
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4461-5. doi: 10.1073/pnas.78.7.4461.
4
The effect of urea on catabolite sensitive operons in Escherichia coli K 12.尿素对大肠杆菌K12中分解代谢敏感操纵子的影响。
Mol Gen Genet. 1980;178(3):611-6. doi: 10.1007/BF00337868.
5
CAP binding to B and Z forms of DNA.CAP与B型和Z型DNA的结合。
Nucleic Acids Res. 1983 Apr 25;11(8):2479-94. doi: 10.1093/nar/11.8.2479.
6
Mechanism for transcriptional action of cyclic AMP in Escherichia coli: entry into DNA to disrupt DNA secondary structure.环磷酸腺苷在大肠杆菌中转录作用的机制：进入DNA以破坏DNA二级结构。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4011-5. doi: 10.1073/pnas.78.7.4011.
7
Cyclic AMP receptor proteins interacts with lactose operator DNA.环磷酸腺苷受体蛋白与乳糖操纵基因DNA相互作用。
Nucleic Acids Res. 1981 Jan 24;9(2):277-92. doi: 10.1093/nar/9.2.277.
8
Interaction of the cAMP receptor protein with the lac promoter.环磷酸腺苷受体蛋白与乳糖操纵子启动子的相互作用。
Nucleic Acids Res. 1980 Feb 25;8(4):759-66.
9
Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae.酿酒酵母中可阻遏酸性磷酸酶合成的显性组成型突变PHOO的特性分析。
J Bacteriol. 1974 Nov;120(2):608-17. doi: 10.1128/jb.120.2.608-617.1974.
10
In vitro transcription of the Escherichia coli histidine operon.大肠杆菌组氨酸操纵子的体外转录
Mol Gen Genet. 1974;132(1):1-12. doi: 10.1007/BF00268225.

本文引用的文献

1
Isolation of the lac repressor.乳糖阻遏蛋白的分离
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8. doi: 10.1073/pnas.56.6.1891.
2
Cyclic AMP as an antagonist of catabolite repression in Escherichia coli.环磷酸腺苷作为大肠杆菌中分解代谢物阻遏的拮抗剂。
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3
Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.大肠杆菌乳糖操纵子区域的转位。I. 乳糖操纵子的倒位及噬菌体φ80介导的乳糖转导
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In vitro repression of RNA synthesis by purified lambda phage repressor.纯化的λ噬菌体阻遏物对RNA合成的体外抑制作用。
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5
Control of transcription in bacteria.细菌中的转录调控
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6
Hybrids of bacteriophages lambda and phi 80: a study of nonvegetative functions.噬菌体λ和φ80的杂种：非营养功能的研究
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8
Catabolite-insensitive revertants of lac promoter mutants.乳糖启动子突变体的分解代谢物不敏感回复突变体
Proc Natl Acad Sci U S A. 1970 Jul;66(3):773-9. doi: 10.1073/pnas.66.3.773.
9
Orientation of transcription of the lac operon and its repressor gene in Escherichia coli.大肠杆菌中乳糖操纵子及其阻遏基因的转录方向
J Mol Biol. 1969 Feb 28;40(1):145-51. doi: 10.1016/0022-2836(69)90303-9.
10
Isolation of pure lac operon DNA.纯乳糖操纵子DNA的分离
Nature. 1969 Nov 22;224(5221):768-74. doi: 10.1038/224768a0.

大肠杆菌乳糖操纵子体外转录的起始与抑制机制

Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli.

作者信息

Eron L, Block R

出版信息

Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828-32. doi: 10.1073/pnas.68.8.1828.

DOI:10.1073/pnas.68.8.1828
PMID:4331560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389302/
Abstract

A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiate lac transcription at the lac promoter. Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to the lac promoter. Results support the idea that the lac promoter is composed of two sites that interact with CAP protein and RNA polymerase holoenzyme. The promoter can be altered by mutation so that holoenzyme alone can initiate lac transcription correctly.

摘要

一种环腺苷酸结合蛋白（CAP蛋白）、环腺苷酸和RNA聚合酶全酶被证明在乳糖操纵子启动子处起始乳糖操纵子的转录。乳糖阻遏物似乎通过阻止RNA聚合酶和/或CAP蛋白与乳糖操纵子启动子结合来控制转录。结果支持这样一种观点，即乳糖操纵子启动子由两个与CAP蛋白和RNA聚合酶全酶相互作用的位点组成。启动子可通过突变而改变，从而使单独的全酶能够正确起始乳糖操纵子的转录。