大肠杆菌乳糖操纵子体外转录的起始与抑制机制
Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli.
作者信息
Eron L, Block R
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828-32. doi: 10.1073/pnas.68.8.1828.
Abstract
A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiate lac transcription at the lac promoter. Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to the lac promoter. Results support the idea that the lac promoter is composed of two sites that interact with CAP protein and RNA polymerase holoenzyme. The promoter can be altered by mutation so that holoenzyme alone can initiate lac transcription correctly.
摘要
一种环腺苷酸结合蛋白（CAP蛋白）、环腺苷酸和RNA聚合酶全酶被证明在乳糖操纵子启动子处起始乳糖操纵子的转录。乳糖阻遏物似乎通过阻止RNA聚合酶和/或CAP蛋白与乳糖操纵子启动子结合来控制转录。结果支持这样一种观点，即乳糖操纵子启动子由两个与CAP蛋白和RNA聚合酶全酶相互作用的位点组成。启动子可通过突变而改变，从而使单独的全酶能够正确起始乳糖操纵子的转录。
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本文引用的文献
1
Isolation of the lac repressor.乳糖阻遏蛋白的分离
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8. doi: 10.1073/pnas.56.6.1891.
2
Cyclic AMP as an antagonist of catabolite repression in Escherichia coli.环磷酸腺苷作为大肠杆菌中分解代谢物阻遏的拮抗剂。
FEBS Lett. 1968 Nov;2(1):57-60. doi: 10.1016/0014-5793(68)80100-0.
3
Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.大肠杆菌乳糖操纵子区域的转位。I. 乳糖操纵子的倒位及噬菌体φ80介导的乳糖转导
J Mol Biol. 1966 Aug;19(2):254-65. doi: 10.1016/s0022-2836(66)80003-7.
4
In vitro repression of RNA synthesis by purified lambda phage repressor.纯化的λ噬菌体阻遏物对RNA合成的体外抑制作用。
Nat New Biol. 1971 Mar 17;230(11):76-80. doi: 10.1038/newbio230076a0.
5
Control of transcription in bacteria.细菌中的转录调控
Nat New Biol. 1971 Jan 20;229(3):69-74. doi: 10.1038/newbio229069a0.
6
Hybrids of bacteriophages lambda and phi 80: a study of nonvegetative functions.噬菌体λ和φ80的杂种：非营养功能的研究
Virology. 1969 Apr;37(4):585-96. doi: 10.1016/0042-6822(69)90276-1.
7
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.一种用于检测固定在膜上的DNA与RNA杂交体的定量分析方法。
J Mol Biol. 1965 Jul;12(3):829-42. doi: 10.1016/s0022-2836(65)80331-x.
8
Catabolite-insensitive revertants of lac promoter mutants.乳糖启动子突变体的分解代谢物不敏感回复突变体
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9
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J Mol Biol. 1969 Feb 28;40(1):145-51. doi: 10.1016/0022-2836(69)90303-9.
10
Isolation of pure lac operon DNA.纯乳糖操纵子DNA的分离
Nature. 1969 Nov 22;224(5221):768-74. doi: 10.1038/224768a0.
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