Eron L, Arditti R, Zubay G, Connaway S, Beckwith J R
Proc Natl Acad Sci U S A. 1971 Jan;68(1):215-8. doi: 10.1073/pnas.68.1.215.
An assay system for the in vitro transcription of the lac operon is described. A protein factor (CAP) and cyclic AMP, which are essential for the lac expression in vivo, also stimulate lac transcription in vitro.
本文描述了一种用于体外转录乳糖操纵子的检测系统。一种蛋白质因子(CAP)和环磷酸腺苷(cAMP),它们在体内对乳糖表达至关重要,在体外也能刺激乳糖转录。