Lindemann C B, Rikmenspoel R
Science. 1972 Jan 21;175(4019):337-8. doi: 10.1126/science.175.4019.337.
Bull sperm are deactivated, losing all motility, when they are impaled or dissected with a microprobe. Loss of activity is due to the creation of a hole or break in the cell membrane. Uncoordinated contractile activity is retained if external adenosine triphosphate and adenosine diphosphate are present. When these substances are in the medium, coordinated wave motion can be initiated in impaled or dissected sperm by bending a segment of the flagellum.
公牛精子在用微探针穿刺或解剖时会失活,失去所有运动能力。活性丧失是由于细胞膜上形成了一个洞或破裂。如果存在外部三磷酸腺苷和二磷酸腺苷,不协调的收缩活动会得以保留。当这些物质存在于培养基中时,通过弯曲鞭毛的一段,可以在穿刺或解剖的精子中引发协调的波动运动。