被刺穿的公牛精子鞭毛的硬度
The stiffness of the flagella of impaled bull sperm.
作者信息
Lindemann C B, Rudd W G, Rikmenspoel R
出版信息
Biophys J. 1973 May;13(5):437-48. doi: 10.1016/S0006-3495(73)85997-1.
Abstract
The elastic rigidity (stiffness) of impaled motionless bull sperm flagella has been determined by a manipulatory technique which permitted direct analytical treatment of the experimental system. The effects of external ATP and ADP were measured. It was found that ATP acts as a plasticizing agent, while ADP does not. The stiffness measured for flagella in a medium without ATP was 15 times greater than the value measured with 10 mM ATP present. The rigor-like stiffness measured with no ATP present is reversible with ATP and seems to be correlated to a transition in the state of the contractile system.
摘要
通过一种允许对实验系统进行直接分析处理的操纵技术，测定了被刺穿的静止公牛精子鞭毛的弹性刚度（硬度）。测量了外部ATP和ADP的作用。发现ATP起到增塑剂的作用，而ADP则不然。在没有ATP的培养基中测得的鞭毛刚度比存在10 mM ATP时测得的值大15倍。在没有ATP的情况下测得的类似强直的刚度可被ATP逆转，并且似乎与收缩系统状态的转变相关。
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